| Romanowsky's blood stain | <technique> Prototype of the eosin-methylene blue stain's for blood smears, using aqueous solutions made of a mixture of methylene blue (saturated) and eosin. Romanowsky-type stain's depend for their action on compounds formed by interaction of methylene blue and eosin; most are of no value if water is present in the alcohol because neutral dyes become precipitated. (05 Mar 2000) |
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| chromate stain | <technique> For lead, a method in which tissues preserved in chromate-containing fixatives, such as Regaud's or Orth's fixatives, precipitate lead as yellow lead chromate crystals; formalin-fixed sections are treated with potassium chromate acidified with acetic acid. (05 Mar 2000) |
| chrome alum haematoxylin-phloxine stain | <technique> A stain used to demonstrate pancreatic islet cells; alpha cells appear red, beta cells blue or unstained. (05 Mar 2000) |
| Roux's stain | <technique> A double stain for diphtheria bacilli which employs crystal violet or dahlia and methyl green. (05 Mar 2000) |
| Ciaccio's stain | <technique> A method for demonstrating complex insoluble intra-cellular lipids using fixation in a formalin-dichromate solution, embedding in paraffin, staining with Sudan III or IV, and examination in aqueous mountant. (05 Mar 2000) |
| phosphotungstic acid stain | <technique> The first general stain used for electron microscopy; a selective stain for extracellular components such as elastin, collagen, and basement membrane mucopolysaccharides; it can be followed by uranyl acetate or lead. Synonym: PTA stain. (05 Mar 2000) |
| Wachstein-Meissel stain | <technique> For calcium-magnesium-ATPase, a method similar to that of Gomori's non-specific acid phosphatase stain, except that incubation is carried out with ATP as substrate at neutral pH; enzyme activity is generally demonstrated at cell membranes. (05 Mar 2000) |
| Warthin-Starry silver stain | <technique> A stain for spirochetes in which preparations are incubated in 1% silver nitrate solution followed by a developer. (05 Mar 2000) |
| picrocarmine stain | <technique> A red crystalline powder derived from a solution of carmine, ammonia, and picric acid which is evaporated, leaving the powder (soluble in water); it produces excellent staining of keratohyaline granules. (05 Mar 2000) |
| picro-Mallory trichrome stain | <technique> A modification of Mallory's trichrome stain that involves the addition of picric acid. (05 Mar 2000) |
| picronigrosin stain | <technique> A solution of nigrosin in picric acid, used for staining connective tissue. (05 Mar 2000) |
| Weigert-Gram stain | <technique> A stain for bacteria in tissues in which sections are stained in alum-haematoxylin, then in eosin, aniline methyl violet, and Lugol's solution. (05 Mar 2000) |
| Weigert's iron haematoxylin stain | <technique> A nuclear staining solution containing haematoxylin, ferric chloride, and hydrochloric acid; useful in combination with von Gieson's stain, especially for demonstrating connective tissue elements or Entamoeba histolytica in sections. (05 Mar 2000) |
| Weigert's stain | <technique> 1. For actinomyces, a staining method using immersion in a dark red orsellin solution in alcohol, then staining in crystal-violet solution. See: iron haematoxylin. 2. For elastin, a staining solution of fuchsin, resorcin, and ferric chloride; elastic fibres stain blue-black. 3. For fibrin, a staining method using solutions of aniline-crystal violet and iodine-potassium iodide, then decolorizing in aniline oil and xylol; the fibrin is stained dark blue. 4. For myelin, a staining method using ferric chloride and haematoxylin; myelin stains deep blue, degenerated portions a light yellowish colour. 5. For neuroglia, a complicated process in which the final treatment is like that for staining fibrin; neuroglia and nuclei stain blue. 6. For reticulum, a silver impregnation technique in which reticulum appears as black, well-defined fibres without beading and with a relatively clear background. (05 Mar 2000) |
| Mowry's colloidal iron stain | <technique> A stain used for demonstrating acid mucopolysaccharides. (05 Mar 2000) |