| acid-fast stain | <technique> A staining technique used to determine the cell wall property of a microorganism. After stained with dye such as hot carbolfuschin, an acid-fast organism, (for example Mycobacterium species) will retain the colour in its cell wall after being washed with acid-alcohol. (13 Nov 1997) |
|---|---|
| acid stain | <technique> A dye in which the anion is the coloured component of the dye molecule, e.g., sodium eosinate (eosin). (05 Mar 2000) |
| Ag-AS stain | <technique> A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin. Synonym: Ag-AS stain. (05 Mar 2000) |
| Albert's stain | <technique> A stain for diphtheria bacilli and their metachromatic granules; contains toluidine blue, methyl green, glacial acetic acid, alcohol, and distilled water. (05 Mar 2000) |
| Altmann's anilin-acid fuchsin stain | <technique> A mixture of picric acid, anilin, and acid fuchsin which stains mitochondria crimson against a yellow background. (05 Mar 2000) |
| auramine O fluorescent stain | <technique> A rapid and accurate technique for Mycobacterium tuberculosis, using auramine O-phenol and a methylene blue counterstain. (05 Mar 2000) |
| basic fuchsin-methylene blue stain | <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have nuclei stained purple; collagen, elastic lamina, and connective tissue are stained blue; mitochondria, myelin, and lipid droplets are stained red; cytoplasm, smooth muscle cells, axoplasm, and chrondroblasts are stained pink. (05 Mar 2000) |
| basic stain | <technique> A dye in which the cation is the coloured component of the dye molecule that binds to anionic groups of nucleic acids (PO4≡) or acidic mucopolysaccharides (e.g., chondroitin sulfate). (05 Mar 2000) |
| Bauer's chromic acid leucofuchsin stain | <technique> A stain for glycogen and fungi utilizing chromic acid as an oxidizing agent of polysaccharides, followed by Schiff's reagent; glycogen and fungi cell walls appear deep red. (05 Mar 2000) |
| Becker's stain | <technique> For spirochetes, a stain applied to thin films fixed in formaldehyde-acetic acid; preparations are treated successively with tannin, carbolic acid, and carbol fuchsin. (05 Mar 2000) |
| Bennhold's Congo red stain | <technique> An amyloid stain useful for amyloid detection in pathologic tissue; gives red staining of amyloid; also induces green birefringence to amyloid under polarised light. (05 Mar 2000) |
| Berg's stain | <technique> A method for staining spermatozoa, utilizing a carbol-fuchsin solution followed by dilute acetic acid and methylene blue; spermatozoa are stained a brilliant red and most other structures appear blue to purple. (05 Mar 2000) |
| Best's carmine stain | <technique> A method for the demonstration of glycogen in tissues. (05 Mar 2000) |
| Bielschowsky's stain | <technique> A method of treating tissues with silver nitrate to demonstrate reticular fibres, neurofibrils, axons, and dendrites. (05 Mar 2000) |
| Biondi-Heidenhain stain | <technique> An obsolete stain for spirochetes, using acid fuchsin and orange G. (05 Mar 2000) |