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Ag-AS stain <technique> A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin.
Synonym: Ag-AS stain.
(05 Mar 2000)
Albert's stain <technique> A stain for diphtheria bacilli and their metachromatic granules; contains toluidine blue, methyl green, glacial acetic acid, alcohol, and distilled water.
(05 Mar 2000)
Altmann's anilin-acid fuchsin stain <technique> A mixture of picric acid, anilin, and acid fuchsin which stains mitochondria crimson against a yellow background.
(05 Mar 2000)
auramine O fluorescent stain <technique> A rapid and accurate technique for Mycobacterium tuberculosis, using auramine O-phenol and a methylene blue counterstain.
(05 Mar 2000)
basic fuchsin-methylene blue stain <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have nuclei stained purple; collagen, elastic lamina, and connective tissue are stained blue; mitochondria, myelin, and lipid droplets are stained red; cytoplasm, smooth muscle cells, axoplasm, and chrondroblasts are stained pink.
(05 Mar 2000)
basic stain <technique> A dye in which the cation is the coloured component of the dye molecule that binds to anionic groups of nucleic acids (PO4&equiv;) or acidic mucopolysaccharides (e.g., chondroitin sulfate).
(05 Mar 2000)
Bauer's chromic acid leucofuchsin stain <technique> A stain for glycogen and fungi utilizing chromic acid as an oxidizing agent of polysaccharides, followed by Schiff's reagent; glycogen and fungi cell walls appear deep red.
(05 Mar 2000)
Becker's stain <technique> For spirochetes, a stain applied to thin films fixed in formaldehyde-acetic acid; preparations are treated successively with tannin, carbolic acid, and carbol fuchsin.
(05 Mar 2000)
Bennhold's Congo red stain <technique> An amyloid stain useful for amyloid detection in pathologic tissue; gives red staining of amyloid; also induces green birefringence to amyloid under polarised light.
(05 Mar 2000)
Berg's stain <technique> A method for staining spermatozoa, utilizing a carbol-fuchsin solution followed by dilute acetic acid and methylene blue; spermatozoa are stained a brilliant red and most other structures appear blue to purple.
(05 Mar 2000)
Best's carmine stain <technique> A method for the demonstration of glycogen in tissues.
(05 Mar 2000)
Bielschowsky's stain <technique> A method of treating tissues with silver nitrate to demonstrate reticular fibres, neurofibrils, axons, and dendrites.
(05 Mar 2000)
Biondi-Heidenhain stain <technique> An obsolete stain for spirochetes, using acid fuchsin and orange G.
(05 Mar 2000)
Birch-Hirschfeld stain <technique> An obsolete stain for demonstrating amyloid, using Bismarck brown and crystal violet; amyloid is usually stained a bright ruby red, whereas the cytoplasm of cells is not stained and nuclei are brown.
(05 Mar 2000)
Borrel's blue stain <technique> A stain for demonstrating spirochetes, treponemes, and Borrelia organisms, using silver oxide (prepared by means of mixing solutions of silver nitrate and sodium bicarbonate) and methylene blue.
(05 Mar 2000)
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