| 4-methylmuconolactone methyl-isomerase | <enzyme> From nocardioform actinomycetes; forms 3-methylmuconolactone Registry number: EC 5.3.3.- Synonym: 4-methyl-2-enelactone methylisomerase, mmlm-isomerase (26 Jun 1999) |
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| 4-methyl sterol demethylase | <chemical> This term is used to describe 10 reactions in the collective processes of monooxygenation, decarboxylation and reduction, which, together account for the conversion of each of the two 4-methyl groups of lanosterol to carbon dioxide Chemical name: demethylase, methyl sterol Synonym: 4,4-dimethylzymosterol monooxygenase, sterol 4-demethylase (26 Jun 1999) |
| 4-toluene sulfonate methyl monooxygenase | <enzyme> From comamonas testosteroni t-2; catalyses the transformation of toluene sulfonate or p-toluate to the corresponding alcohol and the methyl side-chain to the corresponding acid in the presence of o2 and NADH; p- and m- toluates and 4-ethylbenzoate are substrates, whereas p-xylene, toluene and p-cresol are not substrates; enzyme also catalyses the demethylation of 4-methoxybenzoate to 4-hydroxybenzoate and formaldehyde; comprises reductase b and oxygenase m Registry number: EC 1.14.13.- Synonym: tsmoxygenase, tsmos, p-ts methyl-monooxygenase, tsam protein, tsab protein (26 Jun 1999) |
| Abbott's stain | <technique> Spores are stained blue with alkaline methylene blue; bodies of the bacilli become pink with eosin counterstain. (05 Mar 2000) |
| aceto-orcein stain | <technique> A stain used for chromosomes in air-dried or squashed cytologic material. (05 Mar 2000) |
| acid-fast stain | <technique> A staining technique used to determine the cell wall property of a microorganism. After stained with dye such as hot carbolfuschin, an acid-fast organism, (for example Mycobacterium species) will retain the colour in its cell wall after being washed with acid-alcohol. (13 Nov 1997) |
| acid stain | <technique> A dye in which the anion is the coloured component of the dye molecule, e.g., sodium eosinate (eosin). (05 Mar 2000) |
| Ag-AS stain | <technique> A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin. Synonym: Ag-AS stain. (05 Mar 2000) |
| Albert's stain | <technique> A stain for diphtheria bacilli and their metachromatic granules; contains toluidine blue, methyl green, glacial acetic acid, alcohol, and distilled water. (05 Mar 2000) |
| Altmann's anilin-acid fuchsin stain | <technique> A mixture of picric acid, anilin, and acid fuchsin which stains mitochondria crimson against a yellow background. (05 Mar 2000) |
| auramine O fluorescent stain | <technique> A rapid and accurate technique for Mycobacterium tuberculosis, using auramine O-phenol and a methylene blue counterstain. (05 Mar 2000) |
| basic fuchsin-methylene blue stain | <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have nuclei stained purple; collagen, elastic lamina, and connective tissue are stained blue; mitochondria, myelin, and lipid droplets are stained red; cytoplasm, smooth muscle cells, axoplasm, and chrondroblasts are stained pink. (05 Mar 2000) |
| basic stain | <technique> A dye in which the cation is the coloured component of the dye molecule that binds to anionic groups of nucleic acids (PO4≡) or acidic mucopolysaccharides (e.g., chondroitin sulfate). (05 Mar 2000) |
| Bauer's chromic acid leucofuchsin stain | <technique> A stain for glycogen and fungi utilizing chromic acid as an oxidizing agent of polysaccharides, followed by Schiff's reagent; glycogen and fungi cell walls appear deep red. (05 Mar 2000) |
| Becker's stain | <technique> For spirochetes, a stain applied to thin films fixed in formaldehyde-acetic acid; preparations are treated successively with tannin, carbolic acid, and carbol fuchsin. (05 Mar 2000) |
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