| Glenner-Lillie stain | <technique> For pituitary, a modification of Mann's methyl blue-eosin stain which changes the dye proportions, buffering the dye mixture, and staining at 60캜; basophils are stained blue to black, acidophils are dark red, chromophobe granules are gray to pink, and erythrocytes are orange; with modification, the method is also useful for enterochromaffin cells, goblet cells, Paneth cells, and pancreatic islet cells. (05 Mar 2000) |
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| C-banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
| vital stain | <technique> A stain that is taken up by live cells and that can be used to stain, for example: a group of cells in a developing embryo in order to try to determine a fate map. (18 Nov 1997) |
| centromere banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
| von Kossa stain | <technique> A stain for calcium in mineralised tissue, utilizing a silver nitrate solution followed by sodium thiosulfate; calcified bone but not osteoid is stained brown to black. Synonym: Kossa stain. (05 Mar 2000) |
| Golgi's stain | <technique> Any of several methods for staining nerve cells, nerve fibres, and neuroglia using fixation and hardening in formalin-osmic-dichromate combinations for various times, followed by impregnation in silver nitrate. (05 Mar 2000) |
| Gomori-Jones periodic acid-methenamine-silver stain | <technique> A staining method using methenamine silver, periodic acid, gold chloride, haematoxylin, and eosin to delineate basement membrane, reticulin, collagen, and nuclei; used in renal histopathology. See: Rambourg's periodic acid-chromic methenamine-silver stain. (05 Mar 2000) |
| Gomori's chrome alum haematoxylin-phloxine stain | <technique> A technique used to demonstrate cytoplasmic granules, after Bouin's or formalin-Zenker fixatives, using oxidised haematoxylin plus phloxine; in the pancreas, beta cells are blue, alpha and delta cells are red, and zymogen granules are red to unstained; in the pituitary, alpha cells are pink, beta cells and chromophobes are gray-blue, and nuclei are purple to blue. (05 Mar 2000) |
| Gomori's methenamine-silver stain | <technique> Techniques for 1) argentaffin cells: a method using a methenamine-silver solution in combination with gold chloride, sodium thiosulphate, and safranin O; argentaffin granules appear brown-black against a green background; 2) urates: warm sections are treated directly with a hot methenamine-silver solution to produce a blackening of urates; 3) fungi: see Grocott-Gomori methenamine-silver stain; 4) melanin, which reduces silver nitrate. (05 Mar 2000) |
| Gomori's non-specific acid phosphatase stain | <technique> A method in which formalin-fixed frozen sections are incubated in a substrate containing sodium beta-glycerophosphate and lead nitrate at pH 5.0; the insoluble lead phosphate produced is treated with ammonium sulfide to give a black lead sulfide. (05 Mar 2000) |
| Gomori's non-specific alkaline phosphatase stain | <technique> A calcium-cobalt sulfide method using frozen sections or cold acetone-or formalin-fixed paraffin sections, plus sodium beta-glycerophosphate as a substrate at pH 9.0 to 9.5 with Mg++ as activator; calcium ions precipitate the liberated phosphate, cobalt salt replaces the calcium phosphate, and ammonium sulfide converts the product to a black cobalt sulfide. (05 Mar 2000) |
| Gomori's one-step trichrome stain | <technique> A connective tissue stain that uses haematoxylin and a dye mixture containing chromotrope 2R and light green or aniline blue; muscle fibres appear red, collagen is green (or blue if aniline blue is used), and nuclei are blue to black. (05 Mar 2000) |
| Gomori's silver impregnation stain | <technique> A reliable method for reticulin, as an aid in the diagnosis of neoplasm and early cirrhosis of the liver; the staining solution employs silver nitrate, potassium hydroxide, and ammonia water carefully prepared to avoid having silver precipitate. (05 Mar 2000) |
| Goodpasture's stain | <technique> A stain for Gram-negative bacteria, using aniline fuchsin. (05 Mar 2000) |
| Gordon and Sweet stain | <technique> A stain for reticulin, using acidified potassium permanganate, oxalic acid, iron alum, silver nitrate, formaldehyde, gold chloride, and sodium thiosulfate. (05 Mar 2000) |