| Berk Sharp technique | <molecular biology, procedure> A technique of genetic mapping in which mRNA is hybridised with single stranded DNA and the nonhybridised DNA then digested with S1 nuclease, the residual DNA that hybridised with the messenger is then characterised by restriction mapping. (18 Nov 1997) |
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| bone demineralization technique | Removal of mineral constituents or salts from bone or bone tissue. Demineralization is used as a method of studying bone strength and bone chemistry. (12 Dec 1998) |
| radioisotope dilution technique | Method for assessing flow through a system by injection of a known quantity of radionuclide into the system and monitoring its concentration over time at a specific point in the system. (12 Dec 1998) |
| random amplified polymorphic DNA technique | Technique that utilises low-stringency polymerase chain reaction (pcr) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. Rapd technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes. (12 Dec 1998) |
| PAP technique | 1. <gynaecology> Colloquial abbreviation for Papanicolaou's stain. 2. <technique> Peroxidase antiperoxidase method for obtaining an enhanced peroxidase reaction to indicate antibody binding to antigen. In the first stage the material, for example a section, is reacted with a specific antiserum (say rat) against the antigen. In the next stage a large excess of say rabbit antirat immunoglobulin is applied so that only one of the binding sites is bound to the first antibody. Then a rat antiperoxidase antiserum is bound to the second antibody unfilled sites and finally peroxidase is added and binds to the third antiserum before the peroxidase is used to develop a colour reaction. (18 Nov 1997) |
| rebreathing technique | Use of a breathing or anaesthesia circuit in which exhaled air is subsequently inhaled either with or without absorption of CO2 from the exhaled air. (05 Mar 2000) |
| Rebuck skin window technique | An in vivo test of the inflammatory response in which the skin is abraded and a slide applied to the abraded area to permit visualization of leukocyte mobilization. (05 Mar 2000) |
| McGoon's technique | Plastic reconstruction of an incompetent mitral valve, when the incompetence is due to rupture of chordae to the posterior leaflet, by plication of the redundant leaflet. (05 Mar 2000) |
| glucose clamp technique | <technique> Maintenance of a constant blood glucose level by perfusion or infusion with glucose or insulin. It is used for the study of metabolic rates (e.g., in glucose, lipid, amino acid metabolism) at constant glucose concentration. (12 Dec 1998) |
| cellulose tape technique | Use of a piece of transparent cellulose tape applied to a glass slide to obtain perianal samples for identification of pinworm eggs. (05 Mar 2000) |
| Merendino's technique | Plastic reconstruction of an incompetent mitral valve using heavy silk sutures to narrow the annulus in the region of the medial commissure. (05 Mar 2000) |
| microetching technique | A method of roughening the surface of a natural tooth or a dental restoration utilizing a gas-impelled jet of fine abrasive. It enhances the attachment of resin cements or restorative materials to the surface. See: airbrasive technique. (05 Mar 2000) |
| Mohs' fresh tissue chemosurgery technique | Chemosurgery in which superficial cancers are excised after fixation in vivo. (05 Mar 2000) |
| washed field technique | The cutting of cavity preparations in teeth utilizing a constant irrigant which is immediately removed from the mouth by means of a vacuum device. (05 Mar 2000) |
| post and core technique | Use of a metal casting, usually with a post in the pulp or root canal, designed to support and retain an artificial crown. (12 Dec 1998) |