| far Western analysis | Yeast gene, induced by factor, that causes cells to arrest in G1 phase, by interacting with the G1 cyclin, CLN2. (18 Nov 1997) |
|---|---|
| zoo blot analysis | A procedure using Southern blot analysis to test the ability of a nucleic acid probe from one species to hybridise with the DNA fragment of another species. (05 Mar 2000) |
| FISH Analysis | <molecular biology, technique> Use of a DNA or RNA probe todetect the presence of the complementaryDNA sequence in cloned bacterial or cultured eukaryotic cells.Also used for locating geneson chromosomes. The process is: Prepare microscope slide with cells in metaphase of mitosis, Treat slide with a weak base. Thus denaturing the DNA. Pour radioactively labelled probe onto the slide. Expose slide to photographic emulsion for a few days or weeks. Develop emulsion. (13 Oct 1997) |
| flow injection analysis | The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device. (12 Dec 1998) |
| fluctuation analysis | Method used to determine (for example) how many ion channels contribute to the transmembrane current. On the assumption that each channel is either open or shut, the noise in the recorded current can be considered to arise from the statistical fluctuation in the number of channels open and the magnitude of the fluctuation gives an estimate of the conductance of a single channel. (18 Nov 1997) |
| fourier analysis | Analysis based on the mathematical function first formulated by jean-baptiste-joseph fourier in 1807. The function, known as the fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the X-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerised tomography imaging, etc. (12 Dec 1998) |
| frequency analysis | <ecology, statistics> A method of evaluating vegetation in an area by establishing a transect and counting the occurrences of plant species at various sampling points along the transect. (07 Apr 1998) |
| least-squares analysis | A principle of estimation in which the estimates of a set of parameters in a statistical model are those quantities minimizing the sum of squared differences between the observed values of a dependent variable and the values predicted by the model. (12 Dec 1998) |
| linkage analysis | <genetics> Study aimed at establishing linkage between genes. Today linkage analysis serves as a way of gene-hunting and genetic testing. (12 Dec 1998) |
| acetoacetyl-acyl carrier protein synthase | <enzyme> E coli enzyme, that catalyses condensation of malonyl-acyl carrier protein plus acetyl-acyl carrier protein; not inhibited by cerulenin Registry number: EC 2.3.1.- Synonym: acetoacetyl-acp synthase (26 Jun 1999) |
| acid soluble spore protein | <molecular biology> A DNA binding protein in the spores of some bacteria, thought to stabilise the DNA in an A configuration, so protecting it from cleavage by enzymes or UV light. (18 Nov 1997) |
| acute-phase protein | <haematology> These plasma proteins (in addition to fibrinogen) increase 25% or more in response to inflammation and injury are under direct control of interleukin-6 (IL-6) (hepatocyte-stimulating factor). Other proteins which increase are ceruloplasmin, C3 and C4 which increase 50% or more; alpha-1 acid glycoprotein, alpha-1 antitrypsin, haptoglobin and fibrinogen (the major determinant of viscosity 1 ) which increase two- to fourfold; C-reactive protein (CRP) and serum amyloid A which increase several hundred-fold. Despite long-held clinical opinion to the contrary, available data indicate that neither ESR nor measurement of specific acute-phase reactants are useful in excluding underlying infection or inflammation regardless of the pretest probability. These proteins are secreted into the blood in increased or decreased quantities by hepatocytes in response to trauma, inflammation, or disease. They can serve as inhibitors or mediators of the inflammatory processes. Certain acute-phase proteins have been used to diagnose and follow the course of diseases or as tumour markers. See also: amyloid, c-reactive protein, erythrocyte sedimentation rate, viscosity. (25 Jun 1999) |
| acyl-(acyl-carrier-protein)-phospholipid acyltransferase | <enzyme> Catalyses the formation of phosphatidylethanolamine from acyl-acyl carrier protein and 2-acyl-sn-glycero-3-phosphoethanolamine Registry number: EC 2.3.1.40 Synonym: 2-acyl-gpe acyltransferase, 2-acylglycerophosphoethanolamine acyltransferase (26 Jun 1999) |
| acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine acyltransferase | <enzyme> E coli enzyme involved in lipid a biosynthesis; uses beta-hydroxymyristoyl-acyl carrier protein to form udp-3-monoacyl-n-acetylglucosamine; amino acid sequence given in second source Registry number: EC 2.3.1.129 Synonym: udp-aguatransferase, lpxa protein, udp-n-acetylglucosamine-3-acyltransferase, udp-n-acetylglucosamine 3-o-acyltransferase, udp-3-o-(r-3-hydroxymyristoyl)glucosamine-n-acyltransferase, lpxd protein, fira gene product, fira protein (26 Jun 1999) |
| acyl carrier protein | <protein> A small (77 peptides long) protein which binds six other enzymes involved in fatty acid synthesis. It was first isolated in E. Coli bacteria. (09 Oct 1997) |