| fluorescamine | <chemical> A nonfluorescent reagent for the detection of primary amines, peptides and proteins. The reaction products are highly fluorescent. Pharmacological action: indicators and reagents. Chemical name: Spiro(furan-2(3H),1'(3'H)-isobenzofuran)-3,3'-dione, 4-phenyl- (12 Dec 1998) |
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| fluoresce | 1. To produce or exhibit fluorescence. 2. 4-phenyl[furan[2H(3H)-1'-phthalane]-3,3'-dione;a reagent that reacts with amino acids to procude a fluorescing compound. (05 Mar 2000) |
| fluorescein | <chemical> Fluorophore commonly used in microscopy. Fluorescein di acetate can be used as a vital stain or can be conjugated to proteins (particularly antibodies) using isothiocyanate. Excitation is at 365nm and the emitted light is green yellow (450-490nm). The emission spectrum is pH sensitive and fluorescein can therefore be used to measure pH in intracellular compartments. (18 Nov 1997) |
| fluorescein angiography | <procedure> The process of taking X-rays of blood vessels that have been injected with a special dye. The dye allows to person viewing the X-ray to see the blood vessels. (13 Nov 1997) |
| fluorescein eye examination | A special test that allows the examiner to detect cornea abrasions or corneal foreign bodies. An orange dye (fluorescein) is placed into the eye and the eye is illuminated with a cobalt blue light (black light). This serves to highlight any irregularities in the corneal surface. (27 Sep 1997) |
| fluorescein instillation test | A test for patency of the lacrimal system; fluorescein instilled in the conjunctival sac can be recovered from the inferior nasal meatus. Synonym: Jones' test. (05 Mar 2000) |
| fluorescein isothiocyanate | <chemical> A fluorescent chemical derived from fluorescein which is used to label proteins. (25 Jun 1999) |
| fluorescein sodium | A dye used for diagnosis of certain ocular diseases, differentiation or delineation of organ parts in surgery, and determination of circulation time. Synonym: resorcinolphthalein sodium, uranin. (05 Mar 2000) |
| fluorescein string test | A string test used to determine location of a bleeding intestinal lesion in which fluorescein is given intravenously to determine gastrointestinal haemorrhage; if the string fluoresces after removal, it has been contaminated by blood that has appeared since injection of the fluorescein; used to determine location of bleeding lesion. (05 Mar 2000) |
| fluorescein-5-isothiocyanate | <chemical> 3',6'-dihydroxy-5-isothiocyanatospiro(isobenzofuran-1(3h),9'-(9h)xanthen)-3-one. Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques. Pharmacological action: fluorescent dyes. Chemical name: Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato- (12 Dec 1998) |
| fluorescein-conjugated antibody | Refers to an antibody that has joined with a fluorescein molecule. (09 Oct 1997) |
| fluoresceins | <chemical> A family of spiro(isobenzofuran-1(3h),9'-(9h)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays. Pharmacological action: fluorescent dyes. (12 Dec 1998) |
| fluorescence | <chemistry, physics> The emission of one or more photons by a molecule or atom activated by the absorption of a quantum of electro magnetic radiation. Typically the emission, that is of longer wavelength than the excitatory radiation, occurs within 10exp 8 seconds: phosphorescence is a phenomenon with a longer or much longer delay in re radiation. Note that rays, X-rays, UV, visible light and IR radiations may all stimulate fluorescence. (25 Jun 1999) |
| fluorescence energy transfer | <technique> Transfer of energy from one fluorochrome to another. The emission wavelength of the fluorochrome excited by the incident light must approximately match the excitation wavelength of the second fluorochrome. If light at the second emission wavelength is detected, it implies that the two fluorochromes were physically within a few nanometres. Used as a technique to probe protein or cell interactions. (25 Jun 1999) |
| fluorescence immunoassay | <technique> A sensitive technique which uses fluorescein, a fluorescent molecule, to measure the antigen or antibody concentration in a solution. (09 Oct 1997) |