| Jerne technique | A technique for measuring immunocompetence by quantitating the number of splenic antibody-forming cells found in a mouse that has been sensitised to sheep erythrocytes. The number of plaques formed correlates with the number of splenic antibody-forming cells. (05 Mar 2000) |
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| Judkins technique | A method of selective coronary artery catheterization utilizing the standard Seldinger technique through a percutaneous femoral artery puncture. (05 Mar 2000) |
| kleinschmidt monolayer technique | <technique> A method for preparing DNA for study under an electron microscope, the DNA molecules are spread as a thin, positively charged protein film onwater. The film is then transferred to a copper viewing grid andplaced under the microscope. (17 Dec 1997) |
| Ficoll-Hypaque technique | A density-gradient centrifugation technique for separating lymphocytes from other formed elements in the blood; the sample is layered onto a Ficoll-sodium metrizoate gradient of specific density; following centrifugation, lymphocytes are collected from the plasma-Ficoll interface. (05 Mar 2000) |
| flicker fusion frequency technique | A technique of perimetry using the criterion of critical fusion frequency. Synonym: flicker fusion frequency technique. (05 Mar 2000) |
| fluorescent antibody technique | Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (fluorescent antibody technique, direct) or an indirect method, by formation of antigen-antibody complex which is then labelled with fluorescein-conjugated anti-immunoglobulin antibody (fluorescent antibody technique, indirect). The tissue is then examined by fluorescence microscopy. (12 Dec 1998) |
| fluorescent antibody technique, direct | A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (12 Dec 1998) |
| fluorescent antibody technique, indirect | A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labelled with fluorescein-conjugated anti-immunoglobulin antibody. (12 Dec 1998) |
| flush technique | <procedure> A technique for determining the systolic blood pressure in infants; the elevated limb is milked of blood from the hand or foot proximally; the blood pressure cuff is then inflated above the likely systolic pressure and the limb lowered; the cuff pressure is then gradually released until the blanched limb flushes. (05 Mar 2000) |
| long cone technique | The use of a cone distance of 14 inches or more in making oral roentgenographs. (05 Mar 2000) |
| A-DNA | A form of DNA in which the helix is right-handed and the overall appearance is short and broad. (05 Mar 2000) |
| a-form DNA | <molecular biology> One of several forms that can be assumed by a double helix. A-DNA is stable in dehydrated conditions. This form is less common than the dominant form found under physiological conditions -- beta-DNA. This form is also assumed by DNA-RNA hybrid helices and by regions of double-stranded RNA. It is a right-handed helix and is a more compact form than beta-DNA. (09 Oct 1997) |
| antisense DNA | <molecular biology> A synthetic DNA strand that is complementary to a particular strand of target DNA with a complementary sequence of bases. This results in preventing expression of the gene encoded. These proteins can be used to selectively turn off production of certain proteins or block viral genetic instructions, by marking them for destruction by cellular enzymes, in order to prevent the building of new virus or the infection of new cells. (14 Nov 1997) |
| apurinic DNA | <molecular biology> A DNA molecule that has lost adenine and guanine, its purine bases. Apurinic DNA can be produced by treating the DNA with acid. (09 Oct 1997) |
| ATP-dependent DNA strand transferase | <enzyme> From human cell nuclei; catalyses strand exchange between homologous DNA sequences; magnesium dependent, requires ATP hydrolysis Registry number: EC 2.7.7.- Synonym: ATP-dep-DNA-str trnsfase (26 Jun 1999) |