| N6-acetyl-beta-lysine transaminase | <enzyme> Involved in oxidative catabolism of lysine by candida maltosa; forms 2-keto-6-acetamidocaproate Registry number: EC 2.6.1.65 Synonym: n6-acetyllysine transaminase, acl-at, n6-acetyllysine 2-oxoglutarate aminotransferase (26 Jun 1999) |
|---|---|
| N-acetyl-6-hydroxytryptophan oxidase | <enzyme> Phenol oxidase from aspergillus nidulans Registry number: EC 1.14.18.- (26 Jun 1999) |
| N-acetyl-alpha-D-galactosaminyloligosaccharidase | <enzyme> From clostridium perfringens; cleaves oligosaccharides larger than disaccharides bound by o-glycosyl linkages to protein Registry number: EC 3.2.1.- Synonym: nag-oligosaccharidase, n-acetylgalactosamine oligosaccharidase (26 Jun 1999) |
| dithiothreitol tetraacetate S-acetyl esterase | <enzyme> Hydrolyzes dithiothreitol tetraactate to dithiothreitol; involved in the ester-mediated prevention of oxidative stress induced cell damage in liver Registry number: EC 3.1.1.- Synonym: dttac-s-acetyl esterase (26 Jun 1999) |
| 2-oxo-delta(3)-4,5,5--trimethylcyclopentenyl acetyl-CoA monooxygenase | <enzyme> Nadph-dependent baeyer-villiger monooxygenase that catalyses conversion of 2-(2'-acetoxyethyl)cyclohexanone to 7-(2'-acetoxyethyl)-2-oxepanone Registry number: EC 1.14.13.- Synonym: odtac monooxygenase (26 Jun 1999) |
| 4-methylumbelliferyl-tetra-N-acetyl-beta-D-chitotetraoside hydrolase | <enzyme> Not identical to human plasma lysozyme Registry number: EC 3.2.1.- Synonym: mu-tact hydrolase (26 Jun 1999) |
| Ames assay | <procedure> One of a number of procedures used to test substances for likely ability to cause cancer that combines the use of animal tissue to generate active metabolites of the substance with a test for mutagenicity in bacteria. (18 Nov 1997) |
| antibiotic assay | <investigation> A test to determine how sensitive a bacterial or fungal strain is to arange of antibiotics bymeasuring the microbes' ability to grow in astandard dilution of each chemical. (09 Oct 1997) |
| assay | <procedure> The determination of the amount of a particular constituent of a mixture or of the biological or pharmacological potency of a drug. (10 May 1997) |
| bandshift assay | <investigation> An assay for proteins, such as transcription factors, that bind specific DNA sequences. A labelled oligonucleotide corresponding to the recognition sequence is incubated with an appropriate nuclear protein extract and run on a nondenaturing acrylamide gel. Oligonucleotides that have been bound by proteins are retarded relative to those that are unbound. (18 Nov 1997) |
| biological assay | <technique> Once a pharmaceutical protein is isolated from the cells in which it was grown, researchers perform tests to measure the protein's biological activity. It must maintain a certain minimal level of biological activity to be used for animal or clinical testing or, later, for market. Researchers also test to confirm that the isolated protein is identical to the desired protein. (21 Mar 1998) |
| radioimmunoprecipitation assay | Sensitive assay using radiolabelled antigens to detect specific antibodies in serum. The antigens are allowed to react with the serum and then precipitated using a special reagent such as protein a sepharose beads. The bound radiolabelled immunoprecipitate is then commonly analyzed by gel electrophoresis. Radioimmunoprecipitation assay (ripa) is often used as a confirmatory test for diagnosing the presence of HIV antibodies. (12 Dec 1998) |
| radioligand assay | <radiobiology> Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labelled binding reagents (e.g., antibodies, intracellular receptors, plasma binders). (12 Dec 1998) |
| radioreceptor assay | A competitive binding assay in which the binder is a membrane or tissue receptor rather than an antibody. (05 Mar 2000) |
| Raji cell radioimmune assay | For immune complexes; a procedure by which immune complexes adsorbed from a test serum by a standard preparation of lymphoblastoid (Raji) cells are assayed by the capacity to bind 125I-labelled antibody to immunoglobulin. (05 Mar 2000) |