| Sanger Coulson method | The most popular method of DNA sequence determination (c.f. Maxam Gilbert sequencing). Starting with single stranded template DNA, a short complementary primer is annealed and extended by a DNA polymerase. The reaction is split into 4 tubes (called A, C, G or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands. (18 Nov 1997) |
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| Sanger method | The method for the sequencing of DNA employing an enzyme that can polymerase DNA and labelled nucleotides. (05 Mar 2000) |
| Sanger's reagent | A reagent used to combine with the free NH2 group of the NH2-terminal amino acid residue in a peptide, thus marking this residue; the combined forms are known as DNP-proteins, Dnp-aminoacyl, etc., the fluorine having been replaced to leave a dinitrophenyl residue (DNP, Dnp, or N2Ph-) attached to the NH2 group. Synonym: Sanger's reagent. Acronym: FDNB (05 Mar 2000) |
| Sanger, Frederick | <person> English biochemist and twice Nobel laureate, *1918. See: Sanger's reagent, Sanger method. (05 Mar 2000) |
| sangu | <zoology> The Abyssinian ox (Bos or Bibos, Africanus), noted for the great length of its horns. It has a hump on its back. Source: Websters Dictionary (01 Mar 1998) |
| sangui- | Blood, bloody. Origin: G. Sanguis (05 Mar 2000) |
| sanguifacient | Synonym: haemopoietic. Origin: sangui-+ L. Facio, to make (05 Mar 2000) |
| sanguiferous | <physiology> Conveying blood; as, sanguiferous vessels, i. E, the arteries, veins, capillaries. Origin: L. Sanguis blood. Source: Websters Dictionary (01 Mar 1998) |
| sanguification | <physiology> The production of blood; the conversion of the products of digestion into blood; haematosis. Origin: Cf. F. Sanguification. See Sanguify. Source: Websters Dictionary (01 Mar 1998) |
| sanguifier | A producer of blood. Source: Websters Dictionary (01 Mar 1998) |
| sanguifluous | Flowing or running with blood. Origin: L. Sanguis blood + fluere to flow. Source: Websters Dictionary (01 Mar 1998) |
| sanguify | To produce blood from. Origin: L. Sanguis blood + -fy: cf. F. Sanguifier. Source: Websters Dictionary (01 Mar 1998) |
| sanguigenous | Producing blood; as, sanguigenous food. Origin: L. Sanguis + -genous. Source: Websters Dictionary (01 Mar 1998) |
| sanguinaceous | Of a blood-red colour; sanguine. Source: Websters Dictionary (01 Mar 1998) |
| sanguinaria | 1. <botany> A genus of plants of the Poppy family. Sanguinaria Canadensis, or bloodroot, is the only species. It has a perennial rootstock, which sends up a few roundish lobed leaves and solitary white blossoms in early spring. See Bloodroot. 2. The rootstock of the bloodroot, used in medicine as an emetic, etc. Origin: NL. See Sanguinary, &. Source: Websters Dictionary (01 Mar 1998) |