| Giemsa chromosome banding stain | <technique> A unique chromosome staining technique, used in human cytogenetics to identify individual chromosomes, which produces characteristic bands. It utilises acetic acid fixation, air drying, denaturing chromosomes mildly with proteolytic enzymes, salts, heat, detergents, or urea, and finally Giemsa stain; chromosome bands appear similar to those fluorochromed by Q-banding stain. Synonym: Giemsa chromosome banding stain. (05 Mar 2000) |
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| Giemsa stain | <technique> Compound of methylene blue-eosin and methylene blue used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears; also used for chromosomes, sometimes after hydrolyzing the cytologic preparation in hot hydrochloric acid, and for showing chromosome G bands; often used in glycerol-methanol buffer solution. (05 Mar 2000) |
| Maximow's stain | <technique> For bone marrow, an alum-haematoxylin and azure II-eosin stain used to distinguish granulated leukocytes, mast cells, and cartilage. (05 Mar 2000) |
| Mayer's haemalum stain | <technique> A progressive nuclear stain also used as a counterstain. (05 Mar 2000) |
| Mayer's mucicarmine stain | A red stain containing aluminum chloride and carmine; used to detect epithelial mucins and mucin-secreting adenocarcinomas; also used to demonstrate the capsule of Cryptococcus neoformans and other fungi. (05 Mar 2000) |
| Mayer's mucihematein stain | A violet-blue staining fluid containing aluminum chloride and haematein; used to detect connective tissue mucins. (05 Mar 2000) |
| May-Grunwald stain | <technique> A German equivalent of Jenner's stain, used for blood staining and in cytology; often used in combination with Giemsa stain; valuable in demonstrating parasitic flagellates. (05 Mar 2000) |
| PAS stain | <technique> A histochemical technique based on periodic acid oxidation of a substance containing the 1,2-glycol grouping. It is used for staining carbohydrates as the resulting dialdehyde reacts with Schiff reagent to form a coloured product. Substances that can be demonstrated include carbohydrates, mucins, cartilage matrix, collagen, reticulum, basement membranes, fibrin, thyroid colloid, amyloid, glomerular hyaline deposits, and a number of other secretions or tissue constituents. Also used in for staining gels on which glycoproteins have been run. See: periodic acid Schiff reaction Synonym: PAS stain. (22 Sep 2002) |
| Glenner-Lillie stain | <technique> For pituitary, a modification of Mann's methyl blue-eosin stain which changes the dye proportions, buffering the dye mixture, and staining at 60캜; basophils are stained blue to black, acidophils are dark red, chromophobe granules are gray to pink, and erythrocytes are orange; with modification, the method is also useful for enterochromaffin cells, goblet cells, Paneth cells, and pancreatic islet cells. (05 Mar 2000) |
| C-banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
| vital stain | <technique> A stain that is taken up by live cells and that can be used to stain, for example: a group of cells in a developing embryo in order to try to determine a fate map. (18 Nov 1997) |
| centromere banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
| von Kossa stain | <technique> A stain for calcium in mineralised tissue, utilizing a silver nitrate solution followed by sodium thiosulfate; calcified bone but not osteoid is stained brown to black. Synonym: Kossa stain. (05 Mar 2000) |
| Golgi's stain | <technique> Any of several methods for staining nerve cells, nerve fibres, and neuroglia using fixation and hardening in formalin-osmic-dichromate combinations for various times, followed by impregnation in silver nitrate. (05 Mar 2000) |
| Gomori-Jones periodic acid-methenamine-silver stain | <technique> A staining method using methenamine silver, periodic acid, gold chloride, haematoxylin, and eosin to delineate basement membrane, reticulin, collagen, and nuclei; used in renal histopathology. See: Rambourg's periodic acid-chromic methenamine-silver stain. (05 Mar 2000) |