| kleinschmidt monolayer technique | <technique> A method for preparing DNA for study under an electron microscope, the DNA molecules are spread as a thin, positively charged protein film onwater. The film is then transferred to a copper viewing grid andplaced under the microscope. (17 Dec 1997) |
|---|---|
| Ficoll-Hypaque technique | A density-gradient centrifugation technique for separating lymphocytes from other formed elements in the blood; the sample is layered onto a Ficoll-sodium metrizoate gradient of specific density; following centrifugation, lymphocytes are collected from the plasma-Ficoll interface. (05 Mar 2000) |
| flicker fusion frequency technique | A technique of perimetry using the criterion of critical fusion frequency. Synonym: flicker fusion frequency technique. (05 Mar 2000) |
| fluorescent antibody technique | Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (fluorescent antibody technique, direct) or an indirect method, by formation of antigen-antibody complex which is then labelled with fluorescein-conjugated anti-immunoglobulin antibody (fluorescent antibody technique, indirect). The tissue is then examined by fluorescence microscopy. (12 Dec 1998) |
| fluorescent antibody technique, direct | A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (12 Dec 1998) |
| fluorescent antibody technique, indirect | A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labelled with fluorescein-conjugated anti-immunoglobulin antibody. (12 Dec 1998) |
| flush technique | <procedure> A technique for determining the systolic blood pressure in infants; the elevated limb is milked of blood from the hand or foot proximally; the blood pressure cuff is then inflated above the likely systolic pressure and the limb lowered; the cuff pressure is then gradually released until the blanched limb flushes. (05 Mar 2000) |
| long cone technique | The use of a cone distance of 14 inches or more in making oral roentgenographs. (05 Mar 2000) |
| androgen binding protein | A protein secreted by testicular Sertoli cells along with inhibin and mullerian inhibiting substance. Androgen binding protein probably maintains a high concentration of androgen in the seminiferous tubules. (05 Mar 2000) |
| androgen-binding proteins | Carrier proteins produced in the sertoli cells of the testis, secreted into the seminiferous tubules, and transported via the efferent ducts to the epididymis. Participate in the transport of androgens; include also synthetic androgens binding proteins. (12 Dec 1998) |
| antigen-binding site | <immunology> In immune network theory, an idiotope, an antigenic site of an antibody that is responsible for that antibody binding to an antigenic determinant (epitope). Also used of the site on a ligand molecule to which a cell surface receptor binds. (18 Nov 1997) |
| binding | <biochemistry, chemistry, molecular biology> The adherence of molecules to one another, for example, enzymes to substrates, antibodies to antigens, DNA strands to their complementary strands. Binding occurs because the shape and chemical natures of parts of the molecules surfaces are complementary. A common metaphor is the "lock-and-key," used to describe how enzymes fit around their substrate. (14 Nov 1997) |
| binding constant | <chemistry> Reciprocal of dissociation constant. A measure of the extent of a reversible association between two molecular species at equilibrium. (18 Nov 1997) |
| binding energy | <chemistry, radiobiology> The binding energy of a nucleus is the minimum energy required to dissociate it into its component neutrons and protons. Neutron or proton binding energies are those required to remove a neutron or proton, respectively, from a nucleus. Electron binding energy is that required to remove an electron from an atom or a molecule. (16 Dec 1997) |
| binding sites | The reactive parts of a macromolecule that directly participate in its specific combination with another molecule. (12 Dec 1998) |