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gram-positive endospore-forming bacteria <microbiology> Bacteria that form endospores and are gram-positive. Representative genera include bacillus, clostridium, micromonospora, saccharopolyspora, and streptomyces.
(12 Dec 1998)
gram-positive endospore-forming rods <microbiology> Rod-shaped bacteria that form endospores and are gram-positive. Representative genera include bacillus and clostridium.
(12 Dec 1998)
gram-positive rods <microbiology> A large group of rod-shaped bacteria that retains the crystal violet stain when treated by gram's method.
(12 Dec 1998)
Gram's iodine <microbiology> A solution containing iodine and potassium iodide, used in Gram's stain.
(05 Mar 2000)
Gram's stain <microbiology, technique> A method for differential staining of bacteria; smears are fixed by flaming, stained in a solution of crystal violet, treated with iodine solution, rinsed, decolorised, and then counterstained with safranin O.
Gram-positive organisms stain purple black and Gram-negative organisms stain pink; useful in bacterial taxonomy and identification, and also in indicating fundamental differences in cell wall structure.
(05 Mar 2000)
Gram stain <microbiology, procedure> A method of staining bacteria using a violet stain. The gram staining characteristics (denoted as positive or negative) can assist in the identification of the offending bacteria.
A heat fixed bacterial smear is stained with crystal violet (methyl violet), treated with 3% iodine/potassium iodide solution, washed with alcohol and counterstained. The method differentiates bacteria into two main classes, gram-positive and gram-negative. Certain bacteria, notably mycobacteria, that have walls with high lipid content show acid-fast staining the stain resists decolouration in strong acid.
(27 Sep 1997)
Weigert-Gram stain <technique> A stain for bacteria in tissues in which sections are stained in alum-haematoxylin, then in eosin, aniline methyl violet, and Lugol's solution.
(05 Mar 2000)
half-a-gram stain <technique> A lab technique used to detect the presence of members from the bacterial family Legionellaceae in samples of sputum.
(09 Oct 1997)
Stirling's modification of Gram's stain <technique> A stable aniline-crystal violet stain.
(05 Mar 2000)
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