| UPEP | urinary protein electrophoresis; urine protein electrophoresis |
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| ADT | Accepted Dental Therapeutics; adenosine triphosphate; admission, discharge, transfer; agar-gel diffu... |
| AGD | agar gel diffusion; agarose diffusion; alpha-ketoglutarate dehydrogenase |
| AGDD | agar gel double diffusion |
| AGP | acid glycoprotein; agar gel precipitation; azurophil granule protein |
| thin-layer electrophoresis | Electrophoretic migrations (separations) through a thin layer of inert material, such as cellulose, supported on a glass or plastic plate. (05 Mar 2000) |
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| Tiselius electrophoresis cell | The special container in a Tiselius apparatus containing the solution to be analyzed electrophoretically. (05 Mar 2000) |
| electrophoresis | <technique> Separation of ionic molecules, (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field. High resolution techniques normally use a gel support for the fluid phase. Examples of gels used are starch, acrylamide, agarose or mixtures of acrylamide and agarose. Frictional resistance produced by the support causes size, rather than charge alone, to become the major determinant of separation. Smaller molecules with a more negative charge will travel faster and further through the gel toward the anode of an electrophoretic cell when high voltage is applied. Similar molecules will group on the gel. They may be visualised by staining and quantitated, in relative terms, using densitometers which continuously monitor the photometric density of the resulting stain. The electrolyte may be continuous (a single buffer) or discontinuous, where a sample is stacked by means of a buffer discontinuity, before it enters the running gel/ running buffer. The gel may be a single concentration or gradient in which pore size decreases with migration distance. In SDS gel electrophoresis of proteins or electrophoresis of polynucleotides, mobility depends primarily on size and is used to determined molecular weight. In pulse field electrophoresis, two fields are applied alternately at right angles to each other to minimise diffusion mediated spread of large linear polymers. See: electrofocussing, pulse field electrophoresis (01 Dec 1998) |
| electrophoresis, capillary | A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of proteins, nucleic acids, and carbohydrates. (12 Dec 1998) |
| electrophoresis, cellulose acetate | Electrophoresis in which cellulose acetate is the diffusion medium. (12 Dec 1998) |
| electrophoresis, disc | Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones. (12 Dec 1998) |
| electrophoresis, paper | Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used. (12 Dec 1998) |
| zone electrophoresis | <chemistry, procedure> A type of electrophoresis used by physical chemists. In it, the components of a mixture are separated into distinct zones by moving the solution through a porous medium such as filter paper. (06 May 1997) |
| free electrophoresis | Electrophoresis of substances placed in a solution in a U-shaped tube. (05 Mar 2000) |
| lipoprotein electrophoresis | Electrophoretic separation of plasma lipoproteins. (05 Mar 2000) |
| agar | <cell culture, chemical, microbiology> This gelatinous material, an extract from red algae (mainly Gelidium and Gracilaria species), is most frequently used as a culture medium, especially for bacteria. It is also used as a thickener in foods, but humans cannot digest it. (06 May 1997) |
| ascitic agar | A form of serum agar. (05 Mar 2000) |
| bile salt agar | An agar medium containing lactose, peptone, sodium taurocholate, and neutral red, for the growth and isolation of Gram-negative rods. (05 Mar 2000) |
| birdseed agar | Media prepared from Guizottia abyssinica seeds used in culturing and in the presumptive diagnosis of Cryptococcus neoformans. (05 Mar 2000) |
| blood agar | <cell culture> An agar-based medium which hasbeen enriched with sterilised, defibinated blood (sheep, rabbit or horse). It is used for primary plating andsubculturing, especially to determine bacterial haemolysis. (09 Oct 1997) |