| conditioned medium | <cell biology> Cell culture medium that has already been partially used by cells. Although depleted of some components, it is enriched with cell derived material, probably including small amounts of growth factors, such cell conditioned medium will support the growth of cells at much lower density and, mixed with some fresh medium, is therefore useful in cloning. (18 Nov 1997) |
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| Mueller-Hinton medium | An agar-based media composed of beef infusion, casamino acids, and starch useful in the isolation of gonococci and meningococci; the recommended medium for antibacterial susceptibility tests for most common aerobic and facultatively anaerobic bacteria. (05 Mar 2000) |
| contrast medium | A substance that is introduced into or around a structure and, because of the difference in absorption of X-rays by the contrast medium and the surrounding tissues, allows radiographic visualisation of the structure. (18 Nov 1997) |
| culture medium | A substance, either solid or liquid, used for the cultivation, isolation, identification, or storage of microorganisms. Synonym: growth medium, medium, nutrient medium. (05 Mar 2000) |
| HAT medium | <cell biology, cell culture> A selective growth medium for animal tissue cells that contains Hypoxanthine, the folate antagonist Aminopterin (or amethopterin) and Thymine. Used for selection of hybrid somatic cell lines, as in the production of monoclonal antibodies. In HAT medium, cells are forced to use these exogenous bases, via the salvage pathways, as their sole source of purines and pyrimidines. Parental cells lacking enzymes such as HGPRT or TK can be eliminated whilst hybrids grow. (18 Nov 1997) |
| Czapek-Dox medium | A culture medium used for the cultivation of fungus species and for identification of Aspergillus and Penicillium species. Synonym: Czapek-Dox medium. (05 Mar 2000) |
| Czapek's medium | <cell culture> An agar medium used to grow fungi and soil bacteria which feed on decaying organic material (called saprobes). The medium contents include sugars, water, and salt. (09 Oct 1997) |
| xenobiotic medium chain fatty acid - coenzyme A ligase | <enzyme> Partial amino acid sequence of enzyme from bovine liver mitochondria given I first source; has high sequence homology to human and rat sa protein Registry number: EC 6.2.1.- Synonym: xl-i ligase, xl-i carboxylic acid - CoA ligase (26 Jun 1999) |
| high osmolar contrast medium | Ionic water-soluble iodinated contrast media. Synonym: high osmolar contrast medium. (05 Mar 2000) |
| selective medium | <cell culture> A medium which allows the growth of certain types of microorganisms in preference to others. For example: an antibiotic-containing medium allows the growth of only those microorganisms which are resistant to this antibiotic. (09 Oct 1997) |
| separating medium | Any coating which serves to prevent one surface from adhering to another, in dentistry, a material usually applied to a cast to facilitate separation from the resin denture base after curing; a coating on impressions to facilitate removal of the cast. (05 Mar 2000) |
| Simmons' citrate medium | A diagnostic medium used in the differentiation of species of Enterobacteriaceae, based on their ability to utilise sodium citrate as the sole source of carbon. (05 Mar 2000) |
| hypoxanthine-aminopterin-thymine medium | A type of agar growth medium (a selective medium) which is used to select for cells that are able to make their nucleic acids out of hypoxanthine and/or thymine. All cells are prevented from making nucleotides by any other means (due to the presence of aminopterin or other drugs in the medium), so cells which are unable to use hypoxanthine and/or thymine to do this will not grow, multiply, and become visible colonies. This leaves the cells which are being selected for. (09 Oct 1997) |
| stratum griseum medium | See: gray layer of superior colliculus. (05 Mar 2000) |
| nutrient medium | A substance, either solid or liquid, used for the cultivation, isolation, identification, or storage of microorganisms. Synonym: growth medium, medium, nutrient medium. (05 Mar 2000) |