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kleinschmidt monolayer technique <technique> A method for preparing DNA for study under an electron microscope, the DNA molecules are spread as a thin, positively charged protein film onwater. The film is then transferred to a copper viewing grid andplaced under the microscope.
(17 Dec 1997)
Ficoll-Hypaque technique A density-gradient centrifugation technique for separating lymphocytes from other formed elements in the blood; the sample is layered onto a Ficoll-sodium metrizoate gradient of specific density; following centrifugation, lymphocytes are collected from the plasma-Ficoll interface.
(05 Mar 2000)
flicker fusion frequency technique A technique of perimetry using the criterion of critical fusion frequency.
Synonym: flicker fusion frequency technique.
(05 Mar 2000)
fluorescent antibody technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (fluorescent antibody technique, direct) or an indirect method, by formation of antigen-antibody complex which is then labelled with fluorescein-conjugated anti-immunoglobulin antibody (fluorescent antibody technique, indirect). The tissue is then examined by fluorescence microscopy.
(12 Dec 1998)
fluorescent antibody technique, direct A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen.
(12 Dec 1998)
fluorescent antibody technique, indirect A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labelled with fluorescein-conjugated anti-immunoglobulin antibody.
(12 Dec 1998)
flush technique <procedure> A technique for determining the systolic blood pressure in infants; the elevated limb is milked of blood from the hand or foot proximally; the blood pressure cuff is then inflated above the likely systolic pressure and the limb lowered; the cuff pressure is then gradually released until the blanched limb flushes.
(05 Mar 2000)
long cone technique The use of a cone distance of 14 inches or more in making oral roentgenographs.
(05 Mar 2000)
T-cell-rich, B-cell lymphoma <tumour> A B-cell lymphoma in which more than 90% of the cells are of T-cell origin, masking the large cells that form the neoplastic B-cell component.
See: adult T-cell lymphoma.
(05 Mar 2000)
absorption cell A small glass chamber with parallel sides, in which absorption spectra of solutions can be obtained.
(05 Mar 2000)
acid cell One of the cell's of the gastric glands; it lies upon the basement membrane, covered by the chief cell's, and secretes hydrochloric acid that reaches the lumen of the gland through fine intracellular and intercellular canals (canaliculi).
Synonym: acid cell, oxyntic cell.
(05 Mar 2000)
acidophil cell A cell whose cytoplasm or its granules stain with acid dyes.
(05 Mar 2000)
acinar cell Any secreting cell lining an acinus, especially applied to the cell's of the pancreas that furnish pancreatic juice and enzymes to distinguish them from the cell's of ducts and the islets of Langerhans.
Synonym: acinous cell.
(05 Mar 2000)
acinar cell tumour A solid and cystic tumour of the pancreas, occurring in young women; tumour cells contain zymogen granules.
(05 Mar 2000)
acinic cell adenocarcinoma <tumour> An adenocarcinoma arising from secreting cells of a racemose gland, particularly the salivary glands.
Synonym: acinar carcinoma, acinic cell carcinoma, acinose carcinoma, acinous carcinoma.
(05 Mar 2000)
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