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column chromatography A form of partition, adsorption, ion exchange, or affinity chromatography in which one phase is liquid (aqueous) flowing down a column packed with the second phase, a solid; the dissolved substances form a partition between the solid and liquid phases depending on the chemical and physical conditions of each phase; the more strongly adsorbed solutes reach the bottom of the column later than the less strongly adsorbed ones.
(05 Mar 2000)
high-performance liquid chromatography <investigation> A lab technique, a type of column chromatography, which uses a combination of several separation techniques to separate substances at higher resolution. Extremely sharp peaks on the elution profile can be produced with high-performance liquid chromatography (HPLC).
(09 Oct 1997)
high-pressure liquid chromatography <investigation> A lab technique, a type of column chromatography, which uses a combination of several separation techniques to separate substances at higher resolution. Extremely sharp peaks on the elution profile can be produced with high-performance liquid chromatography (HPLC).
(09 Oct 1997)
thin layer chromatography <technique> Chromatography using a thin layer of powdered medium on an inert sheet to support the stationary phase. Faster than paper chromatography, gives higher resolution and requires smaller samples.
(18 Nov 1997)
thin-layer chromatography Chromatography through a thin layer of cellulose or similar inert material supported on a glass or plastic plate.
(05 Mar 2000)
two-dimensional chromatography Paper chromatography in which a spot, located originally in one corner of a sheet, is developed in one direction along one side of the sheet, after which the sheet is rotated 90
liquid-liquid chromatography Chromatography in which both the moving phase and the stationary (or reverse-moving) phase are liquids, as in countercurrent distribution.
(05 Mar 2000)
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