| affinity chromatography |
This was performed at room temperature. A column (volume 600 mL, diameter 10 cm) of 10 carboxydecyl Sepharose was equilibrated with 0.01 m Tris/HCl buffer, pH 7.5. The enzyme preparation ( 1200 mL, clarified by centrifugation if necessary) was loaded at a flow of about l L
출처: dx.doi.org/10.1046/j.1432-1327.1999.00005.x
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| affinity |
The strength of noncovalent chemical binding between two substances as measured by the dissociation constant of the complex. (Basically, the tendency for a chemical to bind to a receptor.)
출처: digitalunion.osu.edu/r2rsummer04/broderick.16/Glos...
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| affinity maturation |
The mechanism during an immune response that produces antibodies with a strong ability to bind to a foreign antigen over time. Affinity maturation is produced by changes in the genes that encode immunoglobulin G (IgG) and by
출처:
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| affinity c. |
that based on a highly specific biologic interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Any of these substances, covalently linked to an insoluble support or immobilized in a gel, may serve as the sorbent allowing the interacting substance to be isolated from relatively impure samples; often a 1000-fold purification can be achieved in one step.
출처: www.mercksource.com/pp/us/cns/cns_health_library.j...
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| affinity m. |
the progressive increase in the affinity of antigen for antibody occurring during the immune response, due to selection of B lymphocytes with receptors having high affinity for the antigen.
출처: www.mercksource.com/pp/us/cns/cns_health_library.j...
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