| Padykula-Herman stain | <technique> For myosin ATPase, a technique similar to that of Gomori's non-specific alkaline phosphatase stain, except that incubation is carried out with ATP as the substrate at pH 9.4 in the absence of Mg++; enzyme activity is demonstrated as blackened deposits in the A band of striated muscle sarcomeres; control tissue sections lacking substrate and containing sulfhydryl inhibitors are necessary. (05 Mar 2000) |
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| Paget-Eccleston stain | <technique> An aldehyde-thionin-PAS-orange G staining technique modified to identify seven different cell types in the anterior pituitary gland. (05 Mar 2000) |
| van Ermengen's stain | <technique> A method for staining flagella which utilises glacial acetic acid, osmic acid, tannic acid, silver nitrate, gallic acid, and potassium acetate. (05 Mar 2000) |
| van Gieson's stain | <technique> A mixture of acid fuchsin in saturated picric acid solution, used in collagen staining. (05 Mar 2000) |
| Rambourg's chromic acid-phosphotungstic acid stain | <technique> A stain for glycoproteins, used with an electron microscope, with which ultrathin tissue sections reveal complex carbohydrates in the same locations as shown by Rambourg's periodic acid-chromic methenamine-silver stain. (05 Mar 2000) |
| Rambourg's periodic acid-chromic methenamine-silver stain | <technique> A stain for glycoproteins, used with an electron microscope, adapted from the Gomori-Jones periodic acid-methenamine-silver stain; it produces silver deposits in mature saccules of the Golgi apparatus, lysosomal vesicles, cell coat, and basement membranes. (05 Mar 2000) |
| malarial pigment stain | <technique> A stain using phloxine-toluidine blue O sequence; malarial pigment and nuclei are bluish, erythrocytes and cytoplasm are red to orange; found in phagocytic cells of the reticuloendothelial system. (05 Mar 2000) |
| Maldonado-San Jose stain | <technique> A staining method for staining pancreatic islet cells, using a phloxine-azure B-haematoxylin sequence; alpha cells are purple, beta cells are violet-blue, delta cells are light blue, and exocrine cells are grayish blue with red secretion granules. (05 Mar 2000) |
| panoptic stain | <technique> A stain in which a Romanowsky-type stain is combined with another stain; such a combination improves the staining of cytoplasmic granules and other bodies. (05 Mar 2000) |
| Papanicolaou's stain | <technique> A complex stain for detecting malignant cells in cervical smears. Contains in separate staining stages (a) haematoxylin, (b) Orange G phosphotungstic acid c) Light green, Bismarck Brown, Eosin and phosphotungstic acid. (18 Nov 1997) |
| Papanicolaou stain | <technique> A multichromatic stain used principally on exfoliated cytologic specimens and based on aqueous haematoxylin with multiple counterstaining dyes in 95% ethyl alcohol, giving great transparency and delicacy of detail; important in cancer screening, especially of gynecologic smears. (05 Mar 2000) |
| G-banding stain | <technique> A unique chromosome staining technique, used in human cytogenetics to identify individual chromosomes, which produces characteristic bands. It utilises acetic acid fixation, air drying, denaturing chromosomes mildly with proteolytic enzymes, salts, heat, detergents, or urea, and finally Giemsa stain; chromosome bands appear similar to those fluorochromed by Q-banding stain. Synonym: Giemsa chromosome banding stain. (05 Mar 2000) |
| Pappenheim's stain | <technique> A method for differentiating tubercle and smegma bacilli; the preparation is stained with hot carbol-fuchsin solution, then treated with an alcoholic solution of rosolic acid and methylene blue to which glycerin is added; tubercle bacilli are stained bright red, but smegma bacilli are decolorised. (05 Mar 2000) |
| paracarmine stain | <technique> A staining fluid consisting of a solution of calcium chloride and carminic acid in 75% alcohol. (05 Mar 2000) |
| Mann's methyl blue-eosin stain | <technique> A stain useful for anterior pituitary and viral inclusion bodies; a mixture of the two dyes stains alpha cell granules red, beta cell granules dark blue, chromophobes gray to pink, colloid red, erythrocytes orange-red, and collagen fibres blue; this method is also useful for enterochromaffin, goblet, Paneth, and pancreatic islet cells; Negri bodies appear red while their nuclei and central granules are blue. (05 Mar 2000) |