| IEM | immuno-electron microscopy; inborn error of metabolism |
|---|---|
| ISEM | immunosorbent electron microscopy |
| PhEEM | photoemission electron microscopy |
| TEM | transmission electron microscope/ microscopy; triethylenemelamine |
| TFM | testicular feminization male; testicular feminization mutation; total fluid movement; transmission e... |
| ratio imaging fluorescence microscopy | <procedure> A method of measurement of intracellular pH or intracellular calcium levels, using a fluorescent probe molecule (see fura-2), in which the two different excitation wavelengths are used and the emitted light levels compared. If emission at one wavelength is sensitive to the intracellular ion level and emission at the other wavelength is not, then standardisation for intracellular probe concentration, efficiency of light collection, inactivation of probe and thickness of cytoplasm can all be performed automatically. (17 Dec 1997) |
|---|---|
| reflection X-ray microscopy | <technique> A method of producing enlarged images by means of X rays. In this method the radiation is totally reflected at glancing incidence from polished concave mirrors or from the curved surfaces of single crystals by Bragg reflection. The problem of aberration corrections still limits the resolution obtainable. (05 Aug 1998) |
| video microscopy | <technique> Microscopy that takes advantage of video as an imaging, image processing, analysing, or controlling device. (05 Aug 1998) |
| phase contrast microscopy | <investigation> A simple nonquantitative form of interference micoscopy of great utility in visualising live cells. Small differences in optical path length due to differences in refractive index and thickness of structures are visualised as differences in light intensity. (18 Nov 1997) |
| microscopy | <technique> The science of the interpretive use, and applications of microscopes. (05 Aug 1998) |
| microscopy, atomic force | Microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. A microcomputer keeps track of the vertical excursions as a function of the position of the probe in the horizontal plane and presents the sample's image. (12 Dec 1998) |
| microscopy, confocal | A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible. (12 Dec 1998) |
| microscopy, fluorescence | Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilises antibodies that are labelled with fluorescent dye. (12 Dec 1998) |
| microscopy, immunoelectron | Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays. (12 Dec 1998) |
| microscopy, interference | Microscopy in which physiological and photometric contrast in the image is influenced or produced by the action of optical components which regulate interference. (12 Dec 1998) |
| microscopy, phase-contrast | A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate. (12 Dec 1998) |
| microscopy, polarization | Microscopy using polarised light in which phenomena due to the preferential orientation of optical properties with respect to the vibration plane of the polarised light are made visible and correlated parameters are made measurable. (12 Dec 1998) |
| microscopy, ultraviolet | Microscopy in which the image is formed by ultraviolet radiation and is displayed and recorded by means of photographic film. (12 Dec 1998) |
| microscopy, video | Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in telepathology. (12 Dec 1998) |
| confocal microscopy | <procedure> A system of (usually) epifluorescence light microscopy in which a fine laser beam of light is scanned over the object through the objective lens. The technique is particularly good at rejecting light from outside the plane of focus and so produces higher effective resolution than is normally achieved. (18 Nov 1997) |