| restriction enzyme |
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded DNA. The enzyme makes two incisions, one through each of the phosphate backbones of the double helix without damaging the bases. The chemical bonds that the enzymes cleave can be reformed by other enzymes known as ligases, so that restriction fragments carved from different chromosomes or genes can be spliced together, provided their ends are complementary (more below). ...
Ãâó: en.wikipedia.org/wiki/Restriction_enzyme
|
|---|---|
| restriction map |
A description of restriction endonuclease cleavage sites within a piece of DNA. Generating such a map is usually the first step in characterizing an unknown DNA, and a prerequisite to manipulating it for other purposes. Typically, restriction enzymes that cleave DNA infrequently (eg those with 6 bp recognition sites) and are relatively inexpensive are used to produce at a map.
Ãâó: www.geocities.com/bioinformaticsweb/genomicglossar...
|
| restriction site |
The specific nucleotide sequence in DNA that is recognized by a type II restriction endonuclease and within which it makes a double-stranded cut. Restriction sites usually comprise four or six base pairs that typically are palindromic (qv), eg, 5
Ãâó: www.fao.org/docrep/003/X3910E/X3910E21.htm
|
| restriction map |
The linear array of restriction endonuclease sites on a DNA molecule. See mapping.
Ãâó: www.fao.org/docrep/003/X3910E/X3910E21.htm
|
| resting pulse |
the pulse rate when a person is not experiencing any physical activity or mental stress
Ãâó: www.american-depot.com/services/resources_gl_r.asp
|