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Drabkin's reagent A solution used in the cyanmethemoglobin method of measuring haemoglobin. It consists of sodium bicarbonate, potassium cyanide, and potassium ferricyanide.
(05 Mar 2000)
Dragendorff reagent A reagent used in the detection of alkaloids.
(05 Mar 2000)
Ilosvay reagent Sulfanilic acid 0.5, dissolved in dilute acetic acid 150, mixed with naphthylamine 1, and dissolved in boiling water 20; the blue sediment which forms is dissolved in dilute acetic acid 150; a few drops of this reagent added to water, saliva, or other fluid to be tested will produce a red colour if nitrites are present.
(05 Mar 2000)
Edlefsen's reagent An alkaline permanganate solution used in the determination of sugar in the urine.
(05 Mar 2000)
Edman reagent Phenyl isothiocyanate. The classic method for sequence determination of peptides using sequential cleavage of the N terminal residue after reaction with Edman reagent. The N terminal amino acid is removed as a phenylthiohydantoin derivative.
(18 Nov 1997)
Edman's reagent C6H5-N==C==S, a reagent that condenses with the free N-terminal amino group of a peptide chain to form a phenylthiohydantoin in the Edman method of identifying N-terminal amino acids.
Synonym: Edman's reagent.
Acronym: PITC
(05 Mar 2000)
Ehrlich's diazo reagent Two solutions, one of sodium nitrite, the other of acidified sulfanilic acid, used in bringing about diazotization.
Synonym: Ehrlich's diazo reagent.
(05 Mar 2000)
Erdmann's reagent A mixture of sulfuric and nitric acids, used in testing alkaloids.
(05 Mar 2000)
Esbach's reagent Picric acid, citric acid, and water (in the proportions 1, 2, and 97) used for the detection of albumin in the urine.
(05 Mar 2000)
Exton reagent 50 g sulfosalicylic acid and 200g Na2SO4-10H2O in a liter of water, used as a test for albumin.
(05 Mar 2000)
Uffelmann's reagent To a 2% solution of phenol in water is added to aqueous ferric chloride until the solution becomes violet in colour; this turns lemon yellow in the presence of lactic acid, assumes an opaline tint in butyric acid, and is decolorised by hydrochloric acid.
(05 Mar 2000)
Fehling's reagent An alkaline copper tartrate solution formerly used for detection of reducing sugars.
Synonym: Fehling's reagent.
(05 Mar 2000)
Feulgen reagent <chemical> A staining substance which specifically stains DNA. Robert Feulgen, in 1914, used this stain to find out that DNA is the hereditary material in cells.
(09 Oct 1997)
Folin's reagent The reaction of amino acids in alkaline solution with 1,2-naphthoquinone-4-sulfonate (Folin's reagent) to yield a red colour; useful for quantitative assay.
Synonym: Folin's reagent.
(05 Mar 2000)
Fouchet's reagent A 25% solution of trichloroacetic acid, containing 0.9% ferric chloride; a drop of the reagent added at the surface line of barium chloride-impregnated filter paper which has been dipped in urine for 10 sec will give a green colour if bilirubin is present.
See: Fouchet's stain.
(05 Mar 2000)
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