| VDE endonuclease | <enzyme> From saccharomyces cerevisiae; recognises an extended sequence 5'-tatsyatgyygggtgy/ggrgaarkmgkkaawgaaawg-3'; involved in nucleic acid rearrangements Registry number: EC 3.1.22.- Synonym: vma1 gene product, pi-scei endonuclease, vma1 protozyme (26 Jun 1999) |
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| vsr endonuclease | <enzyme> From E coli, nicks double-stranded DNA which has been damaged to have thymidine residues mismatched to 2'-deoxyguanosine thus initiating vsp mismatch repair Registry number: EC 3.1.21.- (26 Jun 1999) |
| restriction endonuclease | <enzyme, molecular biology> Class of bacterial enzymes that cut DNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages (host DNA is specifically modified at these sites). Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site. Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends. They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. Coli are designated Eco RI and Eco RII. (10 Mar 1998) |
| restriction enzyme, endonuclease | A protein that recognises specific, short nucleotide sequences and cuts DNA at those sites. Bacteria contain over 400 such enzymes that recognise and cut over 100 different DNA sequences. See restriction enzyme cutting site. (05 Mar 2000) |
| SanDI endonuclease | <enzyme> A type-ii restriction endonuclease; isolated from streptomyces; recognises the internally degenerate heptanucleotide sequence 5'-gggwccc-3' Registry number: EC 3.1.21.- Synonym: sandi enase (26 Jun 1999) |
| Schizosaccharomyces pombe DNA endonuclease | <enzyme> Cleaves the phosphodiester bond immediately 5' of either cyclobutane pyrimidine dimers or 6-4 pyrimidine pyrimidones of uv-damaged DNA Registry number: EC 3.1.25.- Synonym: uv damage endonuclease, s. Pombe DNA endonuclease, uve1 gene product, spde (26 Jun 1999) |
| SegE endonuclease | <enzyme> Site-specific endonuclease from phage t4; similar to i-tevi endonuclease Registry number: EC 3.1.25.- (26 Jun 1999) |
| spleen endonuclease | micrococcal endonuclease |
| splicing endonuclease | <enzyme> Cleaves intervening sequences of precursors trna, which are then joined by an RNA ligase Registry number: EC 3.1.27.- Synonym: pre-trna splicing endonuclease, trna splicing endonuclease, splicing endonuclease (xenopus), 3'-pre-trna endonuclease, trna-splicing endonuclease (26 Jun 1999) |
| nucleate endonuclease | A nuclease (a nucleate oligonucleotidohydrolase) that forms oligonucleotides ending in 5'-phosphates from RNA and DNA; hydrolyzes both double-stranded and single-stranded polynucleic acids. Synonym: nucleate endonuclease. (05 Mar 2000) |
| superhelical DNA endonuclease | <enzyme> Produces single nicks in superhelical DNA; co-purifies with calf thymus terminal deoxynucleotidyl transferase Registry number: EC 3.1.21.- (26 Jun 1999) |
| deoxyinosine 3' endonuclease | <enzyme> Cleaves the second phosphodiester bond 3' to deoxyinosine; active on oligonucleotides with deoxyinosine pairs, deoxyinosine-containing single-stranded DNA, and on DNA containing abasic sites (ap) or urea Registry number: EC 3.1.- Synonym: deoxyinosine 3'-endonuclease (26 Jun 1999) |
| DNA-dependent ATPase-endonuclease | <enzyme> Atpase and endonuclease are part of a single protein from phage t4 infected E coli; atpase is activated by duplex, single-stranded or supercoiled DNA Registry number: EC 3.1.21.- Synonym: atpase-endonuclease (26 Jun 1999) |
| I-TEVI endonuclease | <enzyme> From phage t4; belongs to distinctive class of endonucleases and is independent of DNA sequences encoding the intron core Registry number: EC 3.1.- Synonym: i-tevi intron endonuclease (26 Jun 1999) |
| I-TEVII endonuclease | <enzyme> From phage t4; belongs to distinctive class of endonucleases and is independent of DNA sequences encoding the intron core Registry number: EC 3.1.- Synonym: i-tevii intron endonuclease (26 Jun 1999) |