| chromatography, gel | Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (12 Dec 1998) |
|---|---|
| colloidal gel | A colloid that has developed resistance to flow because of chemical or thermal change. (05 Mar 2000) |
| polyacrilamide gel | A white, water-soluble, solid gel used as a gel base for electrophoresisand as a thickening or adhesive additive in other industrialapplications. (09 Oct 1997) |
| polyacrylamide gel electrophoresis | Analytical and separative technique in which molecules, particularly proteins, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. Commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight. See: SDS PAGE. (21 Jun 1999) |
| pulsed-field gel electrophoresis | Gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules. Synonym: pulsed-field gel electrophoresis. (05 Mar 2000) |
| pulse-field gel electrophoresis | Gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules. Synonym: pulsed-field gel electrophoresis. (05 Mar 2000) |
| sol gel transformation | Transition between more fluid cytoplasm (endoplasm) and stiffer gel like ectoplasm proposed as a mechanism for amoeboid locomotion: since the endoplasm cannot really be considered a simple fluid and has visco elastic properties like a gel, the term is misleading. (18 Nov 1997) |
| disc gel | Confusingly, nothing to do with shape, gels in which there is a discontinuity in pH or gel concentration or buffer composition. (18 Nov 1997) |
| electrophoresis, agar gel | Electrophoresis in which agar or agarose gel is used as the diffusion medium. (12 Dec 1998) |
| electrophoresis, gel, pulsed-field | Electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length. (12 Dec 1998) |
| electrophoresis, gel, two-dimensional | Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels. (12 Dec 1998) |
| electrophoresis, polyacrylamide gel | Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (12 Dec 1998) |
| electrophoresis, starch gel | Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium. (12 Dec 1998) |
| two dimensional gel electrophoresis | <technique> A high resolution separation technique in which protein samples are separated by isoelectric focussing in one dimension and then laid on an SDS gel for size determined separation in the second dimension. Can resolve hundreds of components on a single gel. (18 Nov 1997) |
| acetyl reduction assay | <investigation> A technique for measuring the nitrogen fixation activity in photosynthetic organisms. It uses a flame ionisation detector and a gas chromatography apparatus to determine the reduction of acetylene to ethylene by the enzyme nitrogenase. (06 May 1997) |