| GSC | gas-solid chromatography; gravity settling culture |
|---|---|
| HPAC | high-performance anion-exchange chromatography; hypothalamo-pituitary-adreno-cortical |
| HPLAC | high-pressure liquid-affinity chromatography |
| HRC | hereditary renal cancer; high-resolution chromatography; horse red cell; human rights committee |
| HSLC | high-speed liquid chromatography |
| ion exchange chromatography | <procedure> Separation of molecules by absorption and desorption from charged polymers. An important technique for protein purification. For small molecules the support is usually polystyrene, but for macromolecules, cellulose, acrylamide or agarose supports give less non-specific absorption and denaturation. Typical charged residues are CM carboxymethyl) or DEAE (diethylaminoethyl). (27 Oct 1998) |
|---|---|
| thin layer chromatography | <technique> Chromatography using a thin layer of powdered medium on an inert sheet to support the stationary phase. Faster than paper chromatography, gives higher resolution and requires smaller samples. (18 Nov 1997) |
| thin-layer chromatography | Chromatography through a thin layer of cellulose or similar inert material supported on a glass or plastic plate. (05 Mar 2000) |
| two-dimensional chromatography | Paper chromatography in which a spot, located originally in one corner of a sheet, is developed in one direction along one side of the sheet, after which the sheet is rotated 90 |
| liquid-liquid chromatography | Chromatography in which both the moving phase and the stationary (or reverse-moving) phase are liquids, as in countercurrent distribution. (05 Mar 2000) |
| agar-gel reaction | <immunology> The reaction between an antibody and an antigen during an immunology lab procedure where the two are allowed to diffuse toward each other through an agar-gel medium. Lines of precipitation form in the places on the gel where the two react with each other and shows where the reaction has occurred. (09 Oct 1997) |
| agarose gel electrophoresis | <procedure> A type of electrophoresis that uses a matrix of highly purified agar to separate large nucleotides in size. (06 May 1997) |
| aluminum hydroxide gel | A suspension containing Al2O3, mainly in the form of aluminum hydroxide, used as an antacid; a dried form, with the same use, is obtained by drying the product of interaction in aqueous solution of an aluminum salt with ammonium or sodium carbonate. (05 Mar 2000) |
| aluminum phosphate gel | An aqueous suspension of between 4.0 and 5.0% of aluminum phosphate; used as an antacid. (05 Mar 2000) |
| bag-gel implant | An implant composed of a silicone rubber bag containing a silicone gel; used in augmentation mammaplasty. (05 Mar 2000) |
| gel | Jelly like material formed by the coagulation of a colloidal liquid. Many gels have a fibrous matrix and fluid filled interstices: gels are viscoelastic rather than simply viscous and can resist some mechanical stress without deformation. Examples are the gels formed by large molecules such as collagen (and gelatin), agarose, acrylamide and starch. (18 Nov 1997) |
| gel diffusion | Diffusion in a gel, as in the case of gel diffusion precipitin tests in which the immune reactants diffuse in agar. See: immunodiffusion. (05 Mar 2000) |
| gel diffusion precipitin tests | Precipitin test's in which the immune precipitate forms in a gel medium (usually agar) into which one or both reactants have diffused; generally classified in two types, in one dimension, and in two dimensions. Synonym: gel diffusion reactions. (05 Mar 2000) |
| gel diffusion precipitin tests in one dimension | Precipitin test's in which antigen solution and antibody incorporated in agar are layered in tubes, permitting effective diffusion in the vertical dimension; the antibody-containing agar may be overlaid directly with antigen solution (single (gel) diffusion in one dimension). (05 Mar 2000) |
| gel diffusion precipitin tests in two dimensions | Precipitin test's made in a layer of agar that permits radial diffusion, in both of the horizontal dimensions, of one or both reactants. Double (gel) diffusion in two dimensions (Ouchterlony test, technique, or method) incorporates antigen and antibody solutions placed in separate wells in a sheet of plain agar, permitting radial diffusion of both reactants; this method is widely used to determine antigenic relationships; the bands of precipitate that form where the reactants meet in optimal concentration are of three patterns, referred to as reaction of identity, reaction of partial identity (cross-reaction), and reaction of nonidentity. (05 Mar 2000) |