| fluorescence recovery after photobleaching | Many fluorochromes are bleached by exposure to exciting light. If, for example: the cell surface is labelled with a fluorescent probe and an area bleached by laser illumination, then the bleached patch that starts off as a dark area will gradually recover fluorescence. The recovery is due to the re population of the area by unbleached molecules and diffusion of bleached molecules to other areas. The rate and extent of recovery are a measure of the fluidity of the membrane and the proportion of labelled molecules that are free to exchange with adjacent areas. The technique is usually applied to cell surface fluidity or viscosity measurements, but is also applicable to other structures. (18 Nov 1997) |
|---|---|
| fluorescence spectrum | Fluorescence evoked over a range of wavelengths when the excitation wavelength is at a maximum. (05 Mar 2000) |
| fluorescence-activated cell sorter | <technique> Flow cytometry is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells. Complex computerised instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light. The cells are categorised first by size and then computer analysed to sort the mixture of cellular elements into cell type by size. Cells are labelled with fluorescent dye and then passed, in suspending medium, through a narrow dropping nozzle so that each cell is in a small droplet. A laser based detector system is used to excite fluorescence and droplets with positively fluorescent cells are given an electric charge. Charged and uncharged droplets are separated as they fall between charged plates and so collect in different tubes. The machine can be used either as an analytical tool, counting the number of labelled cells in a population or to separate the cells for subsequent growth of the selected population. Further sophistication can be built into the system by using a second laser system at right angles to the first to look at a second fluorescent label or to gauge cell size on the basis of light scatter. The great strength of the system is that it looks at large numbers of individual cells and makes possible the separation of populations with, for example: particular surface properties. Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal antibody conjugates are utilised, even when the size of the cell is identical to other subset species. Flow cytometry is a slightly imprecise but common term for the use of the Fluorescence-activated Cell Sorter (FACS). (01 Dec 1998) |
| fluorescence-activated cell sorting | <technique> A technique for separating and sorting cells marked with a fluorescent label based on how much they fluoresce at a particular wavelength. (12 Jan 1998) |
| fluorescent | Having the ability to emit light of a certain wavelength when activated by light of another wavelength. (09 Oct 1997) |
| fluorescent antibody | Immunoglobulin molecule which as been coupled with a fluorescent molecule so that it exhibits fluorescence. (09 Oct 1997) |
| fluorescent antibody technique | Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (fluorescent antibody technique, direct) or an indirect method, by formation of antigen-antibody complex which is then labelled with fluorescein-conjugated anti-immunoglobulin antibody (fluorescent antibody technique, indirect). The tissue is then examined by fluorescence microscopy. (12 Dec 1998) |
| fluorescent antibody technique, direct | A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (12 Dec 1998) |
| fluorescent antibody technique, indirect | A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labelled with fluorescein-conjugated anti-immunoglobulin antibody. (12 Dec 1998) |
| fluorescent antibody techniques | Lab techniques for locating antigens in a prepared tissue sample by using antibodies with fluorescent labels which will bind to the antigens of interest. (09 Oct 1997) |
| fluorescent antibody titre | An indirect method of measuring the concentration of an antibody in serum through the detection of fluorescent light emitted in a chemical reaction. (27 Sep 1997) |
| fluorescent antinuclear antibody test | FANA test, a test for antinuclear antibody components; used, in particular, for the diagnosis of collagen-vascular diseases. (05 Mar 2000) |
| fluorescent dyes | Dyes that emit light when exposed to light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. They are used as markers in biochemistry and immunology. (12 Dec 1998) |
| fluorescent in situ hybridization | See FISH. (12 Dec 1998) |
| fluorescent label | A molecule which fluoresces and can be attached to a probe molecule that does not fluoresce. (09 Oct 1997) |
Synonyms :
Synonyms : Coon's Technic, Fluorescent Antibody Technic, Immunofluorescence, Immunofluorescence Technic, Antibody Technic, Fluorescent, Antibody Technics, Fluorescent, Antibody Technique, Fluorescent, Antibody Techniques, Fluorescent, Coon Technic, Coon Technique
Synonyms : Direct Fluorescent Antibody Technic, Direct Fluorescent Antibody Technique, Direct Immunofluorescence, Direct Immunofluorescence Assay, Fluorescent Antibody Technic, Direct, Immunofluorescence Technic, Direct, Assay, Direct Immunofluorescence
Synonyms : Fluorescent Antibody Technic, Indirect, Immunofluorescence Technic, Indirect, Indirect Fluorescent Antibody Technic, Indirect Fluorescent Antibody Technique, Indirect Immunofluorescence, Indirect Immunofluorescence Assay, Assay, Indirect Immunofluorescence
Synonyms : Fluorescence Agents, Fluorescent Agents, Fluorogenic Substrate, Agents, Fluorescence, Agents, Fluorescent, Dyes, Fluorescent, Probes, Fluorescent, Substrate, Fluorogenic
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| fluorescent |
emitting light during exposure to radiation from an external source a lighting fixture that uses a fluorescent lamp brilliantly colored and apparently giving off light; "fluorescent colors"
Ãâó: wordnet.princeton.edu/perl/webwn
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| fluoridation |
the addition of a fluoride to the water supply (to prevent dental decay)
Ãâó: wordnet.princeton.edu/perl/webwn
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| fluorine |
a nonmetallic univalent element belonging to the halogens; usually a yellow irritating toxic flammable gas; a powerful oxidizing agent; recovered from fluorite or cryolite or fluorapatite
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| fluor |
fluorite: a soft mineral (calcium fluoride) that is fluorescent in ultraviolet light; chief source of fluorine
Ãâó: wordnet.princeton.edu/perl/webwn
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| fluorescence microscopy |
light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes
Ãâó: wordnet.princeton.edu/perl/webwn
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| fluor | a halocarbon in which some hydrogen atoms have been replaced by fluorine |
|---|---|
| fluor | a plastic made with fluorocarbon |
| fluor | any of various fluorescent substances used in fluorescence microscopy to stain specimens |
| fluor | colorless gas haloform CHF3 (similar to chloroform) |
| fluor | an X-ray machine that combines an X-ray source and a fluorescent screen to enable direct observation |
| fluor | examination of body structures using a fluoroscope |
| fluor | a pathological condition resulting for an excessive intake of fluorine (usually from drinking water) |
| fluor | an antimetabolite used to treat certain cancers |
| fluor | a soft mineral (calcium fluoride) that is fluorescent in ultraviolet light |
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