| C-banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
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| centromere banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
| reverse banding | <technique> A reverse Giemsa chromosome banding method that produces bands complementary to G-bands; induced by treatment with high temperature, low pH, or acridine orange staining; often used together with G-banding on human karyotype to determine whether there are deletions. (05 Mar 2000) |
| chromosome banding | Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping. (12 Dec 1998) |
| prometaphase banding | Banding done in the stage of mitosis intermediate between prophase and metaphase. (05 Mar 2000) |
| high-resolution banding | Banding, especially in prophase, which increases the clarity and number of discernible chromosome bands. (05 Mar 2000) |
| pulmonary artery banding | A surgical method of decreasing pulmonary blood flow and thereby volume overload of the left ventricle, alleviating CHF in certain congenital heart defects. (05 Mar 2000) |
| NOR-banding | A procedure which utilises a silver stain that preferentially accumulates in the nucleoli-organizing regions, i.e., the satellite regions of the acrocentric chromosomes. (05 Mar 2000) |
| Q-banding | <cell biology, technique> A fluorescent stain for chromosomes which produces specific banding patterns for each pair of homologous chromosomes. The acridine dye derivative, quinacrine hydrochloride, or other derivatives like quinacrine mustard dihydrochloride produces a green-yellow fluorescence at pH 4.5 in chromosome segments rich in constitutive heterochromatin with deoxyadenylate-deoxythymidilate (A-T) bases of DNA. The centromeric regions of human chromosomes 3, 4, and 13 are specifically stained, as are satellites of some acrocentric chromosomes and the end of the long arm of the Y chromosome. Banding patterns are similar to those obtained with G-banding stain and similar fluorescent stain results are seen with the antibiotics adriamycin and daunomycin, as well as the tertiary dyes butyl proflavine and dapi, and the bisbenzimidazole dye hoechst 33258. See: banding patterns, Q bands, quinacrine. Synonym: quinacrine chromosome banding stain. (12 Jul 2000) |
| quinacrine chromosome banding stain | q-banding stain |
| telomeric R-banding stain | <technique> A modified R-banding stain in which the telomeres become strongly stained and faint R-banding still occurs over the rest of the chromosomes; uses air-dried slides, aging for several days, and staining in hot phosphate-buffered Giemsa stain. (05 Mar 2000) |
| Trusler's rule for pulmonary artery banding | A method that gives guidance as to the correct tightness of the band; the degree of banding for a complex congenital cardiac anomaly with bidirectional shunting less than that for simple ones. (05 Mar 2000) |
| trypsin G-banding stain | <technique> A unique chromosome staining technique, used in human cytogenetics to identify individual chromosomes, which produces characteristic bands. It utilises acetic acid fixation, air drying, denaturing chromosomes mildly with proteolytic enzymes, salts, heat, detergents, or urea, and finally Giemsa stain; chromosome bands appear similar to those fluorochromed by Q-banding stain. Synonym: Giemsa chromosome banding stain. (05 Mar 2000) |