| high-pressure liquid chromatography | <investigation> A lab technique, a type of column chromatography, which uses a combination of several separation techniques to separate substances at higher resolution. Extremely sharp peaks on the elution profile can be produced with high-performance liquid chromatography (HPLC). (09 Oct 1997) |
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| ion exchange chromatography | <procedure> Separation of molecules by absorption and desorption from charged polymers. An important technique for protein purification. For small molecules the support is usually polystyrene, but for macromolecules, cellulose, acrylamide or agarose supports give less non-specific absorption and denaturation. Typical charged residues are CM carboxymethyl) or DEAE (diethylaminoethyl). (27 Oct 1998) |
| thin layer chromatography | <technique> Chromatography using a thin layer of powdered medium on an inert sheet to support the stationary phase. Faster than paper chromatography, gives higher resolution and requires smaller samples. (18 Nov 1997) |
| thin-layer chromatography | Chromatography through a thin layer of cellulose or similar inert material supported on a glass or plastic plate. (05 Mar 2000) |
| two-dimensional chromatography | Paper chromatography in which a spot, located originally in one corner of a sheet, is developed in one direction along one side of the sheet, after which the sheet is rotated 90 |
| liquid-liquid chromatography | Chromatography in which both the moving phase and the stationary (or reverse-moving) phase are liquids, as in countercurrent distribution. (05 Mar 2000) |