| Park-Williams fixative | A fixative for spirochetes, comprised of a 2% solution of osmic acid to the fumes of which the bacteria are exposed for a few seconds. (05 Mar 2000) |
|---|---|
| Regaud's fixative | A fixative containing formaldehyde and sodium dichromate, used to preserve mitochondria but not fat; requires afterchroming and extensive washing. (05 Mar 2000) |
| glutaraldehyde fixative | A fixative used in phosphate or cacodylate buffer for electron microscopy, and as a chromatin and enzyme fixative; may be used preceding osmic acid as a second fixative to add membrane preservation for electron microscopy. (05 Mar 2000) |
| Golgi's osmiobichromate fixative | <chemical> An osmic-bichromate mixture used to demonstrate nerve cells and their processes. (05 Mar 2000) |
| Champy's fixative | A mixture of potassium bichromate, chromic acid, and osmic acid, considered an excellent cytologic fixative with advantages and disadvantages similar to those of Flemming's fixative; it differs from Flemming's fixative in substituting bichromate for acetic acid. (05 Mar 2000) |
| methanol fixative | A fixative used with dry blood films, and often incorporated into the stain used. (05 Mar 2000) |
| picroformol fixative | A fixative containing formalin and picric acid. (05 Mar 2000) |
| Muller's fixative | A hardening fixative composed of potassium dichromate, sodium sulfate, and distilled water, similar to Regaud's fixative. (05 Mar 2000) |
| Helly's fixative | A combination of potassium dichromate, mercuric chloride, formaldehyde, and distilled water, used as a microanatomic fixative for cytoplasmic granules and nuclear staining; has the same disadvantages as Zenker's fixative. (05 Mar 2000) |
| Schaudinn's fixative | A solution of mercuric chloride, sodium chloride, alcohol, and glacial acetic acid, used on wet smears for cytologic fixation. (05 Mar 2000) |
| neutral buffered formalin fixative | A general histologic fixative less likely to leave formalin deposits in tissue than formol-saline fixative. (05 Mar 2000) |
| Newcomer's fixative | A fixative containing isopropanol, propionic acid, and dioxane, recommended as a substitute for Carnoy's fixative in preservation of chromatin; also useful for fixing polysaccharides; small pieces of tissue must be used, although excessive shrinkage may still occur. (05 Mar 2000) |
| Orth's fixative | Formalin added to Muller's fixative, used for bringing out chromaffin, studying early degenerative processes and necrosis, and for demonstrating rickettsiae and bacteria. (05 Mar 2000) |
| osmic acid fixative | A fixative used alone in buffer or as a postfixative after a glutaraldehyde fixative in electron microscopy; an excellent membrane fixative but a poor preservative of chromatin. (05 Mar 2000) |
| Thoma's fixative | Nitric acid in 95% alcohol, used for decalcifying bone in the preparation of histologic specimens. (05 Mar 2000) |