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gram-positive endospore-forming bacteria <microbiology> Bacteria that form endospores and are gram-positive. Representative genera include bacillus, clostridium, micromonospora, saccharopolyspora, and streptomyces.
(12 Dec 1998)
gram-positive endospore-forming rods <microbiology> Rod-shaped bacteria that form endospores and are gram-positive. Representative genera include bacillus and clostridium.
(12 Dec 1998)
gram-positive rods <microbiology> A large group of rod-shaped bacteria that retains the crystal violet stain when treated by gram's method.
(12 Dec 1998)
Gram's iodine <microbiology> A solution containing iodine and potassium iodide, used in Gram's stain.
(05 Mar 2000)
Gram's stain <microbiology, technique> A method for differential staining of bacteria; smears are fixed by flaming, stained in a solution of crystal violet, treated with iodine solution, rinsed, decolorised, and then counterstained with safranin O.
Gram-positive organisms stain purple black and Gram-negative organisms stain pink; useful in bacterial taxonomy and identification, and also in indicating fundamental differences in cell wall structure.
(05 Mar 2000)
Gram stain <microbiology, procedure> A method of staining bacteria using a violet stain. The gram staining characteristics (denoted as positive or negative) can assist in the identification of the offending bacteria.
A heat fixed bacterial smear is stained with crystal violet (methyl violet), treated with 3% iodine/potassium iodide solution, washed with alcohol and counterstained. The method differentiates bacteria into two main classes, gram-positive and gram-negative. Certain bacteria, notably mycobacteria, that have walls with high lipid content show acid-fast staining the stain resists decolouration in strong acid.
(27 Sep 1997)
Weigert-Gram stain <technique> A stain for bacteria in tissues in which sections are stained in alum-haematoxylin, then in eosin, aniline methyl violet, and Lugol's solution.
(05 Mar 2000)
half-a-gram stain <technique> A lab technique used to detect the presence of members from the bacterial family Legionellaceae in samples of sputum.
(09 Oct 1997)
Stirling's modification of Gram's stain <technique> A stable aniline-crystal violet stain.
(05 Mar 2000)
Abbott's method A method of treatment of scoliosis by use of a series of plaster jackets applied after partial correction of the curvature by external force.
(05 Mar 2000)
Abell-Kendall method A standard method for estimation of total serum cholesterol involving saponification of cholesterol ester by hydroxide, extraction with petroleum ether, and colour development with acetic anhydride-sulfuric acid; the method avoids interference by bilirubin, protein, and haemoglobin.
(05 Mar 2000)
activated sludge method A method of sewage disposal in which the sewage is treated with 15% bacterially active, liquid sludge, which is produced by repeated vigorous aeration of fresh sewage to form floccules or sediment; when this flocculation process is complete, the resulting activated sludge contains large numbers of bacteria, together with yeasts, molds, and protozoa, which actively effect the oxidation of organic compounds; this mixture is piped to a sedimentation tank, the effluent from which is completely treated sewage.
(05 Mar 2000)
Altmann-Gersh method The method of rapidly freezing a tissue and dehydrating it in a vacuum.
(05 Mar 2000)
Anel's method Ligation of an artery immediately above (on the proximal side of) an aneurysm.
(05 Mar 2000)
Antyllus' method Ligation of the artery above and below an aneurysm, followed by incision into and emptying of the sac.
(05 Mar 2000)
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