| sulphydryl reagent | <chemical, pharmacology> Compounds that bind to SH groups. Include p chlormercuribenzoate, N ethyl maleimide, iodoacetamide. Very important in studies of protein structure. (18 Nov 1997) |
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| diazo reagent | Two solutions, one of sodium nitrite, the other of acidified sulfanilic acid, used in bringing about diazotization. Synonym: Ehrlich's diazo reagent. (05 Mar 2000) |
| Dische reagent | The assay of DNA by means of the blue colour formed with diphenylamine in acid (Dische reagent). (05 Mar 2000) |
| Dische-Schwarz reagent | Reagent used in the colourimetric detection of RNA. (05 Mar 2000) |
| Drabkin's reagent | A solution used in the cyanmethemoglobin method of measuring haemoglobin. It consists of sodium bicarbonate, potassium cyanide, and potassium ferricyanide. (05 Mar 2000) |
| Dragendorff reagent | A reagent used in the detection of alkaloids. (05 Mar 2000) |
| Ilosvay reagent | Sulfanilic acid 0.5, dissolved in dilute acetic acid 150, mixed with naphthylamine 1, and dissolved in boiling water 20; the blue sediment which forms is dissolved in dilute acetic acid 150; a few drops of this reagent added to water, saliva, or other fluid to be tested will produce a red colour if nitrites are present. (05 Mar 2000) |
| Edman reagent | Phenyl isothiocyanate. The classic method for sequence determination of peptides using sequential cleavage of the N terminal residue after reaction with Edman reagent. The N terminal amino acid is removed as a phenylthiohydantoin derivative. (18 Nov 1997) |
| Edman's reagent | C6H5-N==C==S, a reagent that condenses with the free N-terminal amino group of a peptide chain to form a phenylthiohydantoin in the Edman method of identifying N-terminal amino acids. Synonym: Edman's reagent. Acronym: PITC (05 Mar 2000) |
| Ehrlich's diazo reagent | Two solutions, one of sodium nitrite, the other of acidified sulfanilic acid, used in bringing about diazotization. Synonym: Ehrlich's diazo reagent. (05 Mar 2000) |
| Erdmann's reagent | A mixture of sulfuric and nitric acids, used in testing alkaloids. (05 Mar 2000) |
| Esbach's reagent | Picric acid, citric acid, and water (in the proportions 1, 2, and 97) used for the detection of albumin in the urine. (05 Mar 2000) |
| Exton reagent | 50 g sulfosalicylic acid and 200g Na2SO4-10H2O in a liter of water, used as a test for albumin. (05 Mar 2000) |
| Uffelmann's reagent | To a 2% solution of phenol in water is added to aqueous ferric chloride until the solution becomes violet in colour; this turns lemon yellow in the presence of lactic acid, assumes an opaline tint in butyric acid, and is decolorised by hydrochloric acid. (05 Mar 2000) |
| Fehling's reagent | An alkaline copper tartrate solution formerly used for detection of reducing sugars. Synonym: Fehling's reagent. (05 Mar 2000) |