| RT-PCR | reverse transcriptase-polymerase chain reaction |
|---|---|
| DNA | Deoxyribo-Nucleic Acid |
| DDS | damaged disc syndrome; dendrodendritic synaptosome; dental distress syndrome; depressed DNA synthesi... |
| DNA | deoxyribonucleic acid; did not answer |
| G1 | presynthetic gap [phase of cells prior to DNA synthesis] |
| polymerase beta | A class of mammalian DNA polymerases in the nucleus that do not have a role in replication but may function in DNA repair. Synonym: polymerase b. (05 Mar 2000) |
|---|---|
| polymerase chain reaction | <molecular biology, technique> The first practical system for in vitro amplification of DNA and as such one of the most important recent developments in molecular biology. Two synthetic oligonucleotide primers, which are complementary to two regions of the target DNA (one for each strand) to be amplified, are added to the target DNA (that need not be pure), in the presence of excess deoxynucleotides and Taq polymerase, a heat stable DNA polymerase. In a series (typically 30) of temperature cycles, the target DNA is repeatedly denatured (around 90_C), annealed to the primers (typically at 50-60_C) and a daughter strand extended from the primers (72_C). As the daughter strands themselves act as templates for subsequent cycles, DNA fragments matching both primers are amplified exponentially, rather than linearly. The original DNA need thus be neither pure nor abundant and the polymerase chain reaction has accordingly become widely used not only in research, but in clinical diagnostics and forensic science. Acronym: PCR (14 Oct 1997) |
| polymerase g | A class of mammalian DNA polymerases in the mitochondria responsible for replication of the mitochondrial genome. Synonym: polymerase g. (05 Mar 2000) |
| polymerase gamma | A class of mammalian DNA polymerases in the mitochondria responsible for replication of the mitochondrial genome. Synonym: polymerase g. (05 Mar 2000) |
| polymerase, RNA | Enzyme that catalyses (speeds) the polymerization of RNA. Rna polymerase uses preexisting nucleic acid templates and assembles the RNA from ribonucleotides. (12 Dec 1998) |
| poly polymerase | <enzyme> An enzyme that catalyses the addition of adenine residues to the 3' end of pre-messenger RNAs to form the poly(A) tail. (09 Oct 1997) |
| haem polymerase | <enzyme> Malarial enzyme forms haemozoin from haem derived from haemoglobin; no information on mechanism 2/92 Registry number: EC 2.- Synonym: haem polymerase (26 Jun 1999) |
| Pyrostase polymerase | <enzyme> A thermostable polymerase from thermus flavus; has 3' to 5' exonuclease activity Registry number: EC 2.7.7.- (26 Jun 1999) |
| DyNAzyme polymerase | <enzyme> Commercial product from finnzymes used for gene amplification Registry number: EC 2.7.7.- (26 Jun 1999) |
| O-antigen polymerase | <enzyme> Involved in the binding of o-antigen repeat units and in polymerizing into the o-antigen chain; mw 43.7 kD; genbank x71970 Registry number: EC 2.4.1.- Synonym: rfc protein, s. Flexneri, rfc gene product, o-antigen polymerase, shigella, o-antigen polymerase, pseudomonas, rfc protein, pseudomonas, o-antigen polymerase, e. Coli (26 Jun 1999) |
| Taq polymerase | <enzyme, molecular biology> A heat stable DNA polymerase that is normally used in the polymerase chain reaction. It was isolated from Thermobius aquaticus. (18 Nov 1997) |
| Tli polymerase | <enzyme> Used in polymerase chain reactions; contains a 3'-5' proofreading exonuclease activity Registry number: EC 2.7.7.- Synonym: thermococcus litoralis DNA polymerase, vent, vent(exo-) DNA polymerase, vent(r) DNA polymerase (26 Jun 1999) |
| A-DNA | A form of DNA in which the helix is right-handed and the overall appearance is short and broad. (05 Mar 2000) |
| a-form DNA | <molecular biology> One of several forms that can be assumed by a double helix. A-DNA is stable in dehydrated conditions. This form is less common than the dominant form found under physiological conditions -- beta-DNA. This form is also assumed by DNA-RNA hybrid helices and by regions of double-stranded RNA. It is a right-handed helix and is a more compact form than beta-DNA. (09 Oct 1997) |
| antisense DNA | <molecular biology> A synthetic DNA strand that is complementary to a particular strand of target DNA with a complementary sequence of bases. This results in preventing expression of the gene encoded. These proteins can be used to selectively turn off production of certain proteins or block viral genetic instructions, by marking them for destruction by cellular enzymes, in order to prevent the building of new virus or the infection of new cells. (14 Nov 1997) |