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Rambourg's chromic acid-phosphotungstic acid stain <technique> A stain for glycoproteins, used with an electron microscope, with which ultrathin tissue sections reveal complex carbohydrates in the same locations as shown by Rambourg's periodic acid-chromic methenamine-silver stain.
(05 Mar 2000)
Rambourg's periodic acid-chromic methenamine-silver stain <technique> A stain for glycoproteins, used with an electron microscope, adapted from the Gomori-Jones periodic acid-methenamine-silver stain; it produces silver deposits in mature saccules of the Golgi apparatus, lysosomal vesicles, cell coat, and basement membranes.
(05 Mar 2000)
malarial pigment stain <technique> A stain using phloxine-toluidine blue O sequence; malarial pigment and nuclei are bluish, erythrocytes and cytoplasm are red to orange; found in phagocytic cells of the reticuloendothelial system.
(05 Mar 2000)
Maldonado-San Jose stain <technique> A staining method for staining pancreatic islet cells, using a phloxine-azure B-haematoxylin sequence; alpha cells are purple, beta cells are violet-blue, delta cells are light blue, and exocrine cells are grayish blue with red secretion granules.
(05 Mar 2000)
panoptic stain <technique> A stain in which a Romanowsky-type stain is combined with another stain; such a combination improves the staining of cytoplasmic granules and other bodies.
(05 Mar 2000)
Mallory's aniline blue stain <technique> A method especially suitable for studying connective tissue; sections are stained in acid fuchsin, aniline blue-orange G solution, and phosphotungstic acid; fibrils of collagen are blue, fibroglia, neuroglia, and muscle fibres are red, and fibrils of elastin are pink or yellow.
Synonym: Mallory's aniline blue stain, Mallory's triple stain.
(05 Mar 2000)
Mallory's collagen stain <technique> One of a number of staining methods using phosphomolybdic or phosphotungstic acid with an acid stain, such as aniline blue, or with haematoxylin for connective tissue staining.
(05 Mar 2000)
Mallory's iodine stain <technique> Amyloid appears red-brown after Gram's iodine, then violet and blue after flooding with dilute sulfuric acid.
(05 Mar 2000)
Mallory's phloxine stain <technique> A technique based on retention of phloxine by hyaline after overstaining and then decolorizing with lithium carbonate, used in combination with alum haematoxylin to give nuclear staining; hyaline appears red, older hyaline is pink to colourless, amyloid is pale pink, and nuclei are blue-black.
(05 Mar 2000)
Mallory's phosphotungstic acid haematoxylin stain A stain with broad application in cytology and histology; nuclei, mitochrondria, fibrin, neuroglial fibrils, and cross-striations of skeletal and cardiac muscle stain blue; cartilage ground substance, bone reticulum, and elastin appear in shades of yellow-orange and brownish red; also useful for demonstrating abnormal or diseased astrocytes, often in combination with periodic acid-Schiff stain and Luxol fast blue.
Synonym: Mallory's phosphotungstic acid haematoxylin stain.
(05 Mar 2000)
Mallory's stain <technique> For actinomyces, a stain using alum haematoxylin, followed by eosin; immersion in Ehrlich's aniline crystal violet stain, and Weigert's iodine solution; mycelia stain blue and clubs stain red.
For haemofuchsin, sections are stained sequentially in alum haematoxylin and basic fuchsin; the lipofuchsin-like pigment and ceroid stain bright red, nuclei stain blue, while melanin and haemosiderin appear unstained in their natural browns.
(05 Mar 2000)
Mallory's trichrome stain <technique> A method especially suitable for studying connective tissue; sections are stained in acid fuchsin, aniline blue-orange G solution, and phosphotungstic acid; fibrils of collagen are blue, fibroglia, neuroglia, and muscle fibres are red, and fibrils of elastin are pink or yellow.
Synonym: Mallory's aniline blue stain, Mallory's triple stain.
(05 Mar 2000)
Mallory's triple stain <technique> A method especially suitable for studying connective tissue; sections are stained in acid fuchsin, aniline blue-orange G solution, and phosphotungstic acid; fibrils of collagen are blue, fibroglia, neuroglia, and muscle fibres are red, and fibrils of elastin are pink or yellow.
Synonym: Mallory's aniline blue stain, Mallory's triple stain.
(05 Mar 2000)
Papanicolaou's stain <technique> A complex stain for detecting malignant cells in cervical smears.
Contains in separate staining stages (a) haematoxylin, (b) Orange G phosphotungstic acid c) Light green, Bismarck Brown, Eosin and phosphotungstic acid.
(18 Nov 1997)
Papanicolaou stain <technique> A multichromatic stain used principally on exfoliated cytologic specimens and based on aqueous haematoxylin with multiple counterstaining dyes in 95% ethyl alcohol, giving great transparency and delicacy of detail; important in cancer screening, especially of gynecologic smears.
(05 Mar 2000)
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