| ANIA | automated nephelometric immunoassay |
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| CIA | chemiluminescent immunoassay; chymotrypsin inhibitor activity; colony-inhibiting activity; congenita... |
| EIA | electroimmunoassay; enzyme immunoassay; enzyme-linked immunosorbent assay; equine infectious anemia;... |
| ELIA | enzyme-linked immunoassay |
| EMIT | enzyme multiplied immunoassay technique |
| indirect fluorescent antibody | The detection of antibodies to specific antigenic material in the substrate using fluorescent microscopy. Using fluorescent conjugated antibodies which are specific for a particular isotype of antibody, it is possible to distinguish IgG, IgA and IgM isotypes of specific antibodies using indirect fluorescent antibody. This sensitive technique is highly specific in well-trained hands and recent developments in the establishment of internationally recognised standard materials have led to accurate quantitation of antibody concentrations through endpoint titration (the highest serial dilution of specimen at which specific fluorescence remains) and through measuring visual intensity of fluorescence compared to known reference standard material. (05 Mar 2000) |
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| Kasten's fluorescent Feulgen stain | <technique> A fluorescent modification of the Feulgen stain, utilizing any one of a variety of fluorescent basic dyes to which SO2 is added; the brilliant fluorescence makes this method unusually sensitive and adaptable to cytofluorometric quantification of DNA. (05 Mar 2000) |
| Kasten's fluorescent PAS stain | <technique> A fluorescent modification of the periodic acid Schiff stain for polysaccharides which uses one of Kasten's fluorescent Schiff reagents. (05 Mar 2000) |
| Kasten's fluorescent Schiff reagents | Fluorescent analogues of Schiff's reagent which are fluorescent basic dyes lacking acidic side groups and containing one or more primary amine groups; used in cytochemical detection of DNA in Kasten's fluorescent Feulgen stain, polysaccharides in Kasten's fluorescent PAS stain, and proteins in the ninhydrin-Schiff stain; such analogues include acriflavine, auramine O, and flavophosphine N. (05 Mar 2000) |
| fluorescent | Having the ability to emit light of a certain wavelength when activated by light of another wavelength. (09 Oct 1997) |
| fluorescent antibody | Immunoglobulin molecule which as been coupled with a fluorescent molecule so that it exhibits fluorescence. (09 Oct 1997) |
| fluorescent antibody technique | Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (fluorescent antibody technique, direct) or an indirect method, by formation of antigen-antibody complex which is then labelled with fluorescein-conjugated anti-immunoglobulin antibody (fluorescent antibody technique, indirect). The tissue is then examined by fluorescence microscopy. (12 Dec 1998) |
| fluorescent antibody technique, direct | A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (12 Dec 1998) |
| fluorescent antibody technique, indirect | A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labelled with fluorescein-conjugated anti-immunoglobulin antibody. (12 Dec 1998) |
| fluorescent antibody techniques | Lab techniques for locating antigens in a prepared tissue sample by using antibodies with fluorescent labels which will bind to the antigens of interest. (09 Oct 1997) |
| fluorescent antibody titre | An indirect method of measuring the concentration of an antibody in serum through the detection of fluorescent light emitted in a chemical reaction. (27 Sep 1997) |
| fluorescent antinuclear antibody test | FANA test, a test for antinuclear antibody components; used, in particular, for the diagnosis of collagen-vascular diseases. (05 Mar 2000) |
| fluorescent dyes | Dyes that emit light when exposed to light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. They are used as markers in biochemistry and immunology. (12 Dec 1998) |
| fluorescent in situ hybridization | See FISH. (12 Dec 1998) |
| fluorescent label | A molecule which fluoresces and can be attached to a probe molecule that does not fluoresce. (09 Oct 1997) |