| DNA P | DNA polymerase |
|---|---|
| PCR | DNA-polymerase chain reaction |
| DDRT-PCR | Differential Display Reverse Transcriptase Polymerase Chain Reaction |
| DD-PCR | Differential display polymerase chain reaction |
| DDRT-PCR | Differential display reverse transcription polymerase chain reaction |
| MIP1 DNA polymerase | <enzyme> From saccharomyces cerevisiae; has structural similarity with the E coli DNA polymerase i-type enzymes Registry number: EC 2.7.7.- Synonym: mip1 gene product (26 Jun 1999) |
|---|---|
| MS2 polymerase | <enzyme> Used in formation of fusion proteins Registry number: EC 2.7.7.- (26 Jun 1999) |
| poly(3-hydroxyalkenoate)polymerase | <enzyme> Forms polyesters from medium chain length 3-hydroxy fatty acyl-CoA Registry number: EC 2.3.1.- Synonym: pha polymerase, phac1 gene product (26 Jun 1999) |
| poly(A) polymerase | <enzyme> An enzyme that catalyses the formation of a poly(adenylic acid) sequence. (05 Mar 2000) |
| poly-beta-hydroxybutyrate polymerase | <enzyme> 3rd enzyme of the poly-beta-hydroxybutyrate biosynthetic pathway in alcaligens eutrophus; acts via, an acyl s-enzyme intermediate Registry number: EC 2.3.1.- Synonym: phb polymerase, phb synthase, phac gene product, poly-beta-hydroxybutyrate synthetase (26 Jun 1999) |
| polyglycerol phosphate polymerase | <enzyme> Involved in biosynthesis of teichoic acids Registry number: EC 2.7.8.- (26 Jun 1999) |
| polymerase | <enzyme> Enzymes that catalyse the synthesis of nucleic acids on preexisting nucleic acid templates, assembling RNA from ribonucleotides or DNA from deoxyribonucleotides. (14 Oct 1997) |
| polymerase a | A class of mammalian DNA polymerases in the nucleus that function in chromosome replication. Synonym: polymerase a. (05 Mar 2000) |
| polymerase alpha | A class of mammalian DNA polymerases in the nucleus that function in chromosome replication. Synonym: polymerase a. (05 Mar 2000) |
| polymerase b | A class of mammalian DNA polymerases in the nucleus that do not have a role in replication but may function in DNA repair. Synonym: polymerase b. (05 Mar 2000) |
| polymerase beta | A class of mammalian DNA polymerases in the nucleus that do not have a role in replication but may function in DNA repair. Synonym: polymerase b. (05 Mar 2000) |
| polymerase chain reaction | <molecular biology, technique> The first practical system for in vitro amplification of DNA and as such one of the most important recent developments in molecular biology. Two synthetic oligonucleotide primers, which are complementary to two regions of the target DNA (one for each strand) to be amplified, are added to the target DNA (that need not be pure), in the presence of excess deoxynucleotides and Taq polymerase, a heat stable DNA polymerase. In a series (typically 30) of temperature cycles, the target DNA is repeatedly denatured (around 90_C), annealed to the primers (typically at 50-60_C) and a daughter strand extended from the primers (72_C). As the daughter strands themselves act as templates for subsequent cycles, DNA fragments matching both primers are amplified exponentially, rather than linearly. The original DNA need thus be neither pure nor abundant and the polymerase chain reaction has accordingly become widely used not only in research, but in clinical diagnostics and forensic science. Acronym: PCR (14 Oct 1997) |
| polymerase, DNA | Enzyme that catalyses (speeds) the polymerization of DNA. DNA polymerase uses preexisting nucleic acid templates and assembles the DNA from deoxyribonucleotides. (12 Dec 1998) |
| polymerase, DNA or RNA | Enzymes that catalyse the synthesis of nucleic acids on pre-existing nucleic acid templates, assembling RNA from ribonucleotides or DNA from deoxyribonucleotides. (05 Mar 2000) |
| polymerase g | A class of mammalian DNA polymerases in the mitochondria responsible for replication of the mitochondrial genome. Synonym: polymerase g. (05 Mar 2000) |