| HPLAC | high-pressure liquid-affinity chromatography |
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| LAHC | low affinity-high capacity |
| RBA | relative binding affinity; rescue breathing apparatus; right basilar artery; right brachial artery; ... |
| TBA | tertiary butylacetate; testosterone-binding affinity; tetrabutylammonium; thiobarbituric acid; to be... |
| FIO2 | forced inspiratory oxygen; fractional concentration of oxygen in inspired gas |
| affinity chromatography | <investigation> A technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance. The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture. (09 Feb 1998) |
|---|---|
| affinity column | <investigation> A technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance. The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture. (09 Feb 1998) |
| affinity labeling | <technique> A method for tagging molecules, such as enzymes, so that they can be more easily detected and studied. The method works by substituting a synthetic substance which is similar to the substance that the enzyme normally bonds with. (09 Feb 1998) |
| affinity labels | Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids. (12 Dec 1998) |
| affinity tag | <biochemistry, molecular biology> A short amino acid sequence, usually engineered onto the N- or C-terminus of a protein, to make its purification easier. (14 Nov 1997) |
| antibody affinity | A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes. (12 Dec 1998) |
| residual affinity | Secondary forces that enable apparently saturated atoms, ions, or molecules to attract other atoms or groups, causing such phenomena as complex formation, hydration, adsorption, etc. (05 Mar 2000) |
| chromatography, affinity | A chromatographic technique that utilises the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (12 Dec 1998) |
| low affinity platelet factor IV | Cytokine, produced from platelet basic protein, that acts as a growth factor. (18 Nov 1997) |
| alactic oxygen debt | That part of the oxygen debt that is not lactacid oxygen debt; during recovery, stores of ATP and creatine phosphate must be replenished by oxidative metabolism, and a small amount of oxygen is also needed to restore the normal oxyhemoglobin levels throughout the circulating blood. (05 Mar 2000) |
| alveolar-arterial oxygen difference | The difference or gradient between the partial pressure of oxygen in the alveolar spaces and the arterial blood: P(A-a)02. Normally in young adults this value is less than 20 mm Hg. See: alveolar gas equation. (05 Mar 2000) |
| arteriovenous oxygen difference | <physiology> The difference in the oxygen content (in ml per 100 ml blood) between arterial and venous blood. (05 Mar 2000) |
| biochemical oxygen demand | The amount of oxygen aerobicorganisms need to carry out oxidative metabolism in watercontaining organic matter, such as sewage. (09 Oct 1997) |
| carbon-oxygen ligases | <enzyme> Enzymes that catalyze the joining of two molecules by the formation of a carbon-oxygen bond. Registry number: EC 6.1 (12 Dec 1998) |
| carbon-oxygen lyases | <enzyme> Enzymes that catalyze the cleavage of a carbon-oxygen bond by means other than hydrolysis or oxidation. Registry number: EC 4.2 (12 Dec 1998) |