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high-performance liquid chromatography high-pressure liquid chromatography, (HPLC) a type of automated chromatography in which the mobile phase is a liquid, which is forced under high pressure through a column packed with a sorbent. As in gas chromatography, a detector at the end of the column coupled to a chart recorder graphs the sample efflux. Various separation methods, including adsorption, gel filtration, ion-exchange, and partition, are used.
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high energy bond a chemical bond the hydrolysis of which yields high levels of free energy; such bonds involve phosphate (high energy phosphate b.) or sulfur (high energy sulfur b.) or other mixed anhydride types of chemical structures.
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high energy phosphate bond a high energy bond containing phosphate, occurring in ATP, phosphocreatine, phosphoenolpyruvate, and other phosphate-containing high energy compounds; see high energy compounds, under compound. The energy released on hydrolysis of the bond can be transferred, stored, or used to drive metabolic processes such as the synthesis of glycogen from glucose.
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high frequency blood group a group containing over 99 per cent of individuals, who have a type of erythrocyte antigens called public antigens.
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high pressure liquid chromatography Chromatography is a family of analytical chemistry techniques for the separation of mixtures. It involves passing the sample, a mixture which contains the analyte, in the "mobile phase", often in a stream of solvent, through the "stationary phase." The stationary phase retards the passage of the components of the sample. When components pass through the system at different rates they become separated in time, like runners in a marathon. ...
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