| hydropathy plot | <chemistry, investigation> A graph which shows how hydrophobic each amino acid in a polypeptide is versus where it is located on the polypeptide. The hydropathy plot is used to find clusters of hydrophobic amino acids, which could indicate that the polypeptide in question is a transmembrane protein. A transmembrane protein has hydrophilic parts which protrude out on either side of the cellular membrane, and a hydrophobic centre which lies within the membrane. (09 Oct 1997) |
|---|---|
| double-reciprocal plot | <statistics> A graph made to analyse how fast an enzyme can convert its substrate into its product, depending on how much substrate is present (its concentration) and to determine its maximum speed (after which the enzyme does not get any faster no matter how high the concentration of substrate gets), called Vmax. It is a plot of 1/v versus 1/[S], where v=rate of product formation and [S]=the concentration of the substrate. Synonym: Lineweaver-Burk plot, Woolf-Lineweaver-Burk plot. (05 Mar 2000) |
| Eadie-Hofstee plot | <biochemistry> A graphical representation of enzyme kinetic data in which the velocity of the reaction is plotted on the vertical axis as a function of the v/S ratio on the horizontal axis, with S being the initial substrate concentration. The intercept on the ordiate is Vmax, the slope is Km. Preferable to the Lineweaver Burke plot. Synonym: Eadie-Augustinsson plot, Woolf-Eadie-Augustinsson-Hofstee plot. (05 Jul 2000) |
| Lineweaver Burke plot | A plot of 1/v against 1/S for an enzyme catalysed reaction, where v is the initial rate and s the substrate concentration. From the equation: 1/v = 1/Vmax(1+Km/S) the parameters Vmax and Km can be determined. The equation overweights the contribution of the least accurate points and other methods of analysis are preferred. See: Eadie Hofstee plot. (18 Nov 1997) |
| Lineweaver-Burk plot | <statistics> A graph made to analyse how fast an enzyme can convert its substrate into its product, depending on how much substrate is present (its concentration) and to determine its maximum speed (after which the enzyme does not get any faster no matter how high the concentration of substrate gets), called Vmax. It is a plot of 1/v versus 1/[S], where v=rate of product formation and [S]=the concentration of the substrate. Synonym: Lineweaver-Burk plot, Woolf-Lineweaver-Burk plot. (05 Mar 2000) |