| FA | 1) Fatty Acid 2) Fluorescent Antibodies; 형광 항체 |
|---|---|
| FTA-ABS test | Fluorescent Treponemal Antibody ABSorption test |
| IFA test | Indirect Fluorescent Antibody test |
| CLF | cardiolipin fluorescent [antibody]; ceroid lipofuscinosis; cholesterol-lecithin flocculation |
| DEAFF | detection of early antigen fluorescent foci |
| dyes | Coloured chemical substances that impart more or less permanent colour to other materials. They are used for staining and colouring, as test reagents, and as therapeutic agents in medicine. (12 Dec 1998) |
|---|---|
| oxazin dyes | Similar to azin dye's except that one of the connecting N atoms is replaced by O; most important representatives are brilliant cresyl blue, orcein, litmus, and cresyl violet. (05 Mar 2000) |
| thiazin dyes | Similar to azin dye's except that one of the connecting N atoms is replaced by S; includes many important biological stains, especially in haematology, e.g., azure A, azure B, and methylene blue. (05 Mar 2000) |
| triphenylmethane dyes | A group of dye's that includes pararosanilin, as well as many others used in histology and cytology; employed as nuclear, cytoplasmic, and connective tissue stains; important in histochemistry as in the preparation of Schiff's reagent. (05 Mar 2000) |
| ketonimine dyes | Dye's in which the chromophore is ==C==NH connected to two benzene rings; alkylamino groups are added para to the methane carbon on both rings. The most important member for biological purposes is auramine O; an alternative formulation is as a diphenylmethane dye. (05 Mar 2000) |
| lissamine green dyes | Green dyes containing ammonium and aryl sulfonate moieties that facilitate the visualization of tissues, if given intravenously. They have mostly been used in the study of kidney physiology. (12 Dec 1998) |
| auramine O fluorescent stain | <technique> A rapid and accurate technique for Mycobacterium tuberculosis, using auramine O-phenol and a methylene blue counterstain. (05 Mar 2000) |
| green fluorescent protein | <protein> A protein found in jellyfish which fluoresces, or glows green visible light when excited by UV light with a wavelength of 395 nanometres. It can function as a biological marker when attached to other proteins. The structure of the protein is cylindrical with the glowing component, an amino acid complex called a fluorophore, in the middle of it. (09 Oct 1997) |
| microscope, fluorescent | A microscope equipped to examine material that fluoresces under ultraviolet (uv) light. (12 Dec 1998) |
| direct fluorescent antibody | The straightforward detection of antigens using fluorescent labelled antigen-specific antibody. Because detection of the antigen in a substrate of patient sample (cellular smear, fluid or patient-inoculated culture medium) is the goal, direct fluorescent antibody is seldom quantitative. (05 Mar 2000) |
| direct fluorescent antibody test | Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (fluorescent antibody technique, direct) or an indirect method, by formation of antigen-antibody complex which is then labelled with fluorescein-conjugated anti-immunoglobulin antibody (fluorescent antibody technique, indirect). The tissue is then examined by fluorescence microscopy. (12 Dec 1998) |
| indirect fluorescent antibody | The detection of antibodies to specific antigenic material in the substrate using fluorescent microscopy. Using fluorescent conjugated antibodies which are specific for a particular isotype of antibody, it is possible to distinguish IgG, IgA and IgM isotypes of specific antibodies using indirect fluorescent antibody. This sensitive technique is highly specific in well-trained hands and recent developments in the establishment of internationally recognised standard materials have led to accurate quantitation of antibody concentrations through endpoint titration (the highest serial dilution of specimen at which specific fluorescence remains) and through measuring visual intensity of fluorescence compared to known reference standard material. (05 Mar 2000) |
| Kasten's fluorescent Feulgen stain | <technique> A fluorescent modification of the Feulgen stain, utilizing any one of a variety of fluorescent basic dyes to which SO2 is added; the brilliant fluorescence makes this method unusually sensitive and adaptable to cytofluorometric quantification of DNA. (05 Mar 2000) |
| Kasten's fluorescent PAS stain | <technique> A fluorescent modification of the periodic acid Schiff stain for polysaccharides which uses one of Kasten's fluorescent Schiff reagents. (05 Mar 2000) |
| Kasten's fluorescent Schiff reagents | Fluorescent analogues of Schiff's reagent which are fluorescent basic dyes lacking acidic side groups and containing one or more primary amine groups; used in cytochemical detection of DNA in Kasten's fluorescent Feulgen stain, polysaccharides in Kasten's fluorescent PAS stain, and proteins in the ninhydrin-Schiff stain; such analogues include acriflavine, auramine O, and flavophosphine N. (05 Mar 2000) |