| MGG | May-Grunwald-Giemsa [staining]; molecular and general genetics; mouse gammaglobulin; multinucleated ... |
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| AHI | active hostility index; Animal Health Institute; apnea-plus-hypopnea index |
| CONPA-DRI | III conpa-dri I plus intensified doxorubicin |
| DM-R | decayed plus missing teeth, minus replaced teeth |
| 4E | four-plus edema |
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| fluorescence immunoassay | <technique> A sensitive technique which uses fluorescein, a fluorescent molecule, to measure the antigen or antibody concentration in a solution. (09 Oct 1997) |
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| fluorescence in situ hybridization | <molecular biology, technique> A type of in situ hybridization in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei. Acronym: FISH (25 Jun 1999) |
| fluorescence microscope | <instrument, microscopy> A microscope illuminated by ultraviolet or blue light so that the object may re-radiate light of longer wavelengths. To protect the eyes, a W-absorbing filter should be provided if not built into the fluorescence microscope. (05 Aug 1998) |
| fluorescence microscopy | <procedure> Any type of microscopy in which intrinsic or applied reagents are visualised. Intrinsic fluorescence is often referred to as auto fluorescence. The applied reagents typically include fluorescently labelled proteins that are reactive with sites in the specimen. In particular, fluorescently labelled antibodies are widely used to detect particular antigens in biological specimens. (18 Nov 1997) |
| fluorescence polarisation immunoassay | A technique which takes advantage of the increased polarisation (non-random propagation of emission) of fluorescent light emissions when a fluorescent labelled antigen is bound by reagent antibody. The higher the concentration of unlabelled patient antigen present in the test mixture, the less bound fluorescent antigen is present and, consequently, the lower the polarisation of the fluorescent light emission. Standard calibration yields quantitative results. (05 Mar 2000) |
| fluorescence polarization | Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction. (12 Dec 1998) |
| fluorescence polarization immunoassay | Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labelled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations. (12 Dec 1998) |
| fluorescence recovery after photobleaching | Many fluorochromes are bleached by exposure to exciting light. If, for example: the cell surface is labelled with a fluorescent probe and an area bleached by laser illumination, then the bleached patch that starts off as a dark area will gradually recover fluorescence. The recovery is due to the re population of the area by unbleached molecules and diffusion of bleached molecules to other areas. The rate and extent of recovery are a measure of the fluidity of the membrane and the proportion of labelled molecules that are free to exchange with adjacent areas. The technique is usually applied to cell surface fluidity or viscosity measurements, but is also applicable to other structures. (18 Nov 1997) |
| fluorescence spectrum | Fluorescence evoked over a range of wavelengths when the excitation wavelength is at a maximum. (05 Mar 2000) |
| Abbott's stain | <technique> Spores are stained blue with alkaline methylene blue; bodies of the bacilli become pink with eosin counterstain. (05 Mar 2000) |
| aceto-orcein stain | <technique> A stain used for chromosomes in air-dried or squashed cytologic material. (05 Mar 2000) |
| acid-fast stain | <technique> A staining technique used to determine the cell wall property of a microorganism. After stained with dye such as hot carbolfuschin, an acid-fast organism, (for example Mycobacterium species) will retain the colour in its cell wall after being washed with acid-alcohol. (13 Nov 1997) |
| acid stain | <technique> A dye in which the anion is the coloured component of the dye molecule, e.g., sodium eosinate (eosin). (05 Mar 2000) |
| Ag-AS stain | <technique> A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin. Synonym: Ag-AS stain. (05 Mar 2000) |
| Albert's stain | <technique> A stain for diphtheria bacilli and their metachromatic granules; contains toluidine blue, methyl green, glacial acetic acid, alcohol, and distilled water. (05 Mar 2000) |
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