| fluorescence microscope | <instrument, microscopy> A microscope illuminated by ultraviolet or blue light so that the object may re-radiate light of longer wavelengths. To protect the eyes, a W-absorbing filter should be provided if not built into the fluorescence microscope. (05 Aug 1998) |
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| fluorescence microscopy | <procedure> Any type of microscopy in which intrinsic or applied reagents are visualised. Intrinsic fluorescence is often referred to as auto fluorescence. The applied reagents typically include fluorescently labelled proteins that are reactive with sites in the specimen. In particular, fluorescently labelled antibodies are widely used to detect particular antigens in biological specimens. (18 Nov 1997) |
| fluorescence plus Giemsa stain | <technique> A stain used to demonstrate sister chromatid exchange; cells are grown in 5-bromodeoxyuridine, followed by chromosome preparation, staining in Hoechst 33258, exposure to light, and staining in Giemsa; chromosomes exhibit a "harlequin" appearance. (05 Mar 2000) |
| fluorescence polarisation immunoassay | A technique which takes advantage of the increased polarisation (non-random propagation of emission) of fluorescent light emissions when a fluorescent labelled antigen is bound by reagent antibody. The higher the concentration of unlabelled patient antigen present in the test mixture, the less bound fluorescent antigen is present and, consequently, the lower the polarisation of the fluorescent light emission. Standard calibration yields quantitative results. (05 Mar 2000) |
| fluorescence polarization | Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction. (12 Dec 1998) |
| fluorescence polarization immunoassay | Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labelled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations. (12 Dec 1998) |
| fluorescence recovery after photobleaching | Many fluorochromes are bleached by exposure to exciting light. If, for example: the cell surface is labelled with a fluorescent probe and an area bleached by laser illumination, then the bleached patch that starts off as a dark area will gradually recover fluorescence. The recovery is due to the re population of the area by unbleached molecules and diffusion of bleached molecules to other areas. The rate and extent of recovery are a measure of the fluidity of the membrane and the proportion of labelled molecules that are free to exchange with adjacent areas. The technique is usually applied to cell surface fluidity or viscosity measurements, but is also applicable to other structures. (18 Nov 1997) |
| fluorescence spectrum | Fluorescence evoked over a range of wavelengths when the excitation wavelength is at a maximum. (05 Mar 2000) |
| fluorescence-activated cell sorter | <technique> Flow cytometry is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells. Complex computerised instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light. The cells are categorised first by size and then computer analysed to sort the mixture of cellular elements into cell type by size. Cells are labelled with fluorescent dye and then passed, in suspending medium, through a narrow dropping nozzle so that each cell is in a small droplet. A laser based detector system is used to excite fluorescence and droplets with positively fluorescent cells are given an electric charge. Charged and uncharged droplets are separated as they fall between charged plates and so collect in different tubes. The machine can be used either as an analytical tool, counting the number of labelled cells in a population or to separate the cells for subsequent growth of the selected population. Further sophistication can be built into the system by using a second laser system at right angles to the first to look at a second fluorescent label or to gauge cell size on the basis of light scatter. The great strength of the system is that it looks at large numbers of individual cells and makes possible the separation of populations with, for example: particular surface properties. Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal antibody conjugates are utilised, even when the size of the cell is identical to other subset species. Flow cytometry is a slightly imprecise but common term for the use of the Fluorescence-activated Cell Sorter (FACS). (01 Dec 1998) |
| fluorescence-activated cell sorting | <technique> A technique for separating and sorting cells marked with a fluorescent label based on how much they fluoresce at a particular wavelength. (12 Jan 1998) |
| fluorescent | Having the ability to emit light of a certain wavelength when activated by light of another wavelength. (09 Oct 1997) |
| fluorescent antibody | Immunoglobulin molecule which as been coupled with a fluorescent molecule so that it exhibits fluorescence. (09 Oct 1997) |
| fluorescent antibody technique | Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (fluorescent antibody technique, direct) or an indirect method, by formation of antigen-antibody complex which is then labelled with fluorescein-conjugated anti-immunoglobulin antibody (fluorescent antibody technique, indirect). The tissue is then examined by fluorescence microscopy. (12 Dec 1998) |
| fluorescent antibody technique, direct | A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (12 Dec 1998) |
| fluorescent antibody technique, indirect | A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labelled with fluorescein-conjugated anti-immunoglobulin antibody. (12 Dec 1998) |
Synonyms : Anisotropies, Fluorescence, Fluorescence Anisotropies, Fluorescence Polarizations, Polarizations, Fluorescence
Synonyms : Fluorescence Polarization Immunoassays, Fluoroimmunoassay, Polarization, Fluoroimmunoassays, Polarization, Immunoassays, Fluorescence Polarization, Polarization Fluoroimmunoassays, Polarization Immunoassay, Fluorescence
Synonyms : FRAP (Fluorescence Recovery After Photobleaching), FRAPs (Fluorescence Recovery After Photobleaching)
Synonyms :
Synonyms : Coon's Technic, Fluorescent Antibody Technic, Immunofluorescence, Immunofluorescence Technic, Antibody Technic, Fluorescent, Antibody Technics, Fluorescent, Antibody Technique, Fluorescent, Antibody Techniques, Fluorescent, Coon Technic, Coon Technique
| fluorescence |
Fluorescence is a luminescence, i.e. optical phenomenon in cold bodies, in which a molecule absorbs a high-energy photon, and re-emits it as a lower-energy (longer-wavelength) photon. The energy difference between the absorbed and emitted photons ends up as molecular vibrations (heat). Usually the absorbed photon is in the ultraviolet, and the emitted light (luminescence) is in the visible range, but this depends on the absorbance curve and Stokes shift of the particular fluorophore. ...
출처: en.wikipedia.org/wiki/Fluorescence
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| fluorescent |
Fluorescence is a luminescence, i.e. optical phenomenon in cold bodies, in which a molecule absorbs a high-energy photon, and re-emits it as a lower-energy (longer-wavelength) photon. The energy difference between the absorbed and emitted photons ends up as molecular vibrations (heat). Usually the absorbed photon is in the ultraviolet, and the emitted light (luminescence) is in the visible range, but this depends on the absorbance curve and Stokes shift of the particular fluorophore. ...
출처: en.wikipedia.org/wiki/Fluorescent
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| fluorescein |
(flu
출처: www.merckmedicus.com/pp/us/hcp/thcp_dorlands_conte...
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| fluorescein sodium |
[USP] an odorless, water-soluble, orange-red powder used in dilute solution to reveal corneal trauma, in contact lens fitting, and in retinal angiography. Called also uranin.
출처: www.merckmedicus.com/pp/us/hcp/thcp_dorlands_conte...
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| fluorescence |
The emission of radiation associated with the relaxation of an atom or molecule from an excited energy level to a lower (usually ground state) level. The emission can be in the visible or ultraviolet if an electronic transition is involved, or in the infrared if it is a vibrational transition. See also resonance fluorescence, laser-induced fluorescence, luminescence.
출처: amsglossary.allenpress.com/glossary/browse
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