| directional cloning | DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either endof each restriction fragment. This allows the insert to be ligated tothe vector in a specific orientation and prevents the vector fromrecircularising. (09 Oct 1997) |
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| DNA cloning | The use of DNA manipulation procedures to produce multiple copies of a single gene or segment of DNA. (12 Dec 1998) |
| ambisense expression strategy | <molecular biology> The coding of viral proteins in both the sense (coding) and antisense (noncoding) strands of complementary mRNA, so that the viral proteins are produced no matter which strand gets translated. (09 Oct 1997) |
| mammalian expression vector | <molecular biology> A vector that will produce large amounts of eukaryotic protein taxonomy notwithstanding, and not necessarily a protein from a mammal. (20 Mar 1998) |
| gene expression | <molecular biology> The full use of the information in a gene via transcription and translation leading to production of a protein and hence the appearance of the phenotype determined by that gene. Gene expression is assumed to be controlled at various points in the sequence leading to protein synthesis and this control is thought to be the major determinant of cellular differentiation in eukaryotes. (18 Nov 1997) |
| gene expression regulation | Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action at the level of transcription or translation. These processes include gene activation and genetic induction. (12 Dec 1998) |
| gene expression regulation, archaeal | Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in archaea. (12 Dec 1998) |
| gene expression regulation, bacterial | Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria. (12 Dec 1998) |
| gene expression regulation, developmental | Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism. (12 Dec 1998) |
| gene expression regulation, enzymologic | Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis. (12 Dec 1998) |
| gene expression regulation, fungal | Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi. (12 Dec 1998) |
| gene expression regulation, leukaemic | Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in leukaemia. (12 Dec 1998) |
| gene expression regulation, neoplastic | Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. (12 Dec 1998) |
| gene expression regulation, plant | Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants. (12 Dec 1998) |
| gene expression regulation, viral | Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses. (12 Dec 1998) |