| vehic | vehicle |
|---|---|
| VRT | vehicle rescue technician; volume-rendering technique |
| positional cloning | Cloning a gene based simply on knowing its position in the genome without any idea of the function of that gene. Because this is the reverse of how things have been traditionally done, it has also been called reverse genetics. (12 Dec 1998) |
|---|---|
| self cloning | <molecular biology> Any system in which inappropriate cell types or organisms are eliminated because they possess some character that allows them to die or to remove themselves from the system. Thus a transfected cell with genetic material including a drug resistance marker will be self cloning in the presence of the drug and nontransfected cells will die. (18 Nov 1997) |
| dilution cloning | Cloning by diluting the cell suspension to the point at which the probability of there being more than one cell in the inoculum volume is small. Inevitably on quite a few occasions there will not be any cells. (18 Nov 1997) |
| directional cloning | DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either endof each restriction fragment. This allows the insert to be ligated tothe vector in a specific orientation and prevents the vector fromrecircularising. (09 Oct 1997) |
| DNA cloning | The use of DNA manipulation procedures to produce multiple copies of a single gene or segment of DNA. (12 Dec 1998) |
| expression cloning | <molecular biology> A vector that results in the expression of inserted DNA sequences when propagated in a suitable host cell, i.e. The protein coded for by the DNA is synthesised by the host's system. (06 Aug 1998) |