| hill plot | A method which is used to find out how many binding sites of a particular type are present on a given protein molecule. The Hill plot does this by graphical representation of the molecule. (09 Oct 1997) |
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| Hogness box | <molecular biology> A consensus sequence found in the promoter region of most genes transcribed by eukaryotic RNA polymerase II. Found about 25 nucleotides before the site of initiation of transcription and has the consensus sequence: 5' TATAAAA 3'. This sequence seems to be important in determining accurately the position at which transcription is initiated. (18 Nov 1997) |
| Pu box | <molecular biology> Purine rich sequence recognised by the product of the Sp 1 oncogene. (18 Nov 1997) |
| hydropathy plot | <chemistry, investigation> A graph which shows how hydrophobic each amino acid in a polypeptide is versus where it is located on the polypeptide. The hydropathy plot is used to find clusters of hydrophobic amino acids, which could indicate that the polypeptide in question is a transmembrane protein. A transmembrane protein has hydrophilic parts which protrude out on either side of the cellular membrane, and a hydrophobic centre which lies within the membrane. (09 Oct 1997) |
| snuff-box | See: anatomical snuffbox. (05 Mar 2000) |
| DEAD box helicase | <enzyme> Family of ATP dependent DNA or RNA helicases with a 4 amino acid consensus, D E A D, that resembles an ATP binding site. Examples, p68, a human nuclear protein involved in cell growth, vasa, a Drosophila protein required for specification of posterior embryonic structures. (18 Nov 1997) |
| double-reciprocal plot | <statistics> A graph made to analyse how fast an enzyme can convert its substrate into its product, depending on how much substrate is present (its concentration) and to determine its maximum speed (after which the enzyme does not get any faster no matter how high the concentration of substrate gets), called Vmax. It is a plot of 1/v versus 1/[S], where v=rate of product formation and [S]=the concentration of the substrate. Synonym: Lineweaver-Burk plot, Woolf-Lineweaver-Burk plot. (05 Mar 2000) |
| TATA box | <molecular biology> A consensus sequence found in the promoter region of most genes transcribed by eukaryotic RNA polymerase II. Found about 25 nucleotides before the site of initiation of transcription and has the consensus sequence: 5' TATAAAA 3'. This sequence seems to be important in determining accurately the position at which transcription is initiated. (18 Nov 1997) |
| Eadie-Hofstee plot | <biochemistry> A graphical representation of enzyme kinetic data in which the velocity of the reaction is plotted on the vertical axis as a function of the v/S ratio on the horizontal axis, with S being the initial substrate concentration. The intercept on the ordiate is Vmax, the slope is Km. Preferable to the Lineweaver Burke plot. Synonym: Eadie-Augustinsson plot, Woolf-Eadie-Augustinsson-Hofstee plot. (05 Jul 2000) |
| Lineweaver Burke plot | A plot of 1/v against 1/S for an enzyme catalysed reaction, where v is the initial rate and s the substrate concentration. From the equation: 1/v = 1/Vmax(1+Km/S) the parameters Vmax and Km can be determined. The equation overweights the contribution of the least accurate points and other methods of analysis are preferred. See: Eadie Hofstee plot. (18 Nov 1997) |
| Lineweaver-Burk plot | <statistics> A graph made to analyse how fast an enzyme can convert its substrate into its product, depending on how much substrate is present (its concentration) and to determine its maximum speed (after which the enzyme does not get any faster no matter how high the concentration of substrate gets), called Vmax. It is a plot of 1/v versus 1/[S], where v=rate of product formation and [S]=the concentration of the substrate. Synonym: Lineweaver-Burk plot, Woolf-Lineweaver-Burk plot. (05 Mar 2000) |
| box plot |
The box plot is a method, often used in statistics, to investigate data variation. For every experiment there is a bar chart representing the minimum, maximum, median, 1st quartile and 3rd quartile over all probes of a specific probe list.
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