| IF test | Immuno-Fluorescence test |
|---|---|
| IFA | Immuno-Fluorescence Antibody Test(= Assay) |
| FACS | Fellow of the American College of Surgeons; fluorescence-activated cell sorter |
| FFC | fixed flexion contracture; fluorescence flow cytometry; free from chlorine |
| FIF | feedback inhibition factor; fibroblast interferon; forced inspiratory flow; formaldehyde-induced flu... |
| fluorescence plus Giemsa stain | <technique> A stain used to demonstrate sister chromatid exchange; cells are grown in 5-bromodeoxyuridine, followed by chromosome preparation, staining in Hoechst 33258, exposure to light, and staining in Giemsa; chromosomes exhibit a "harlequin" appearance. (05 Mar 2000) |
|---|---|
| fluorescence polarisation immunoassay | A technique which takes advantage of the increased polarisation (non-random propagation of emission) of fluorescent light emissions when a fluorescent labelled antigen is bound by reagent antibody. The higher the concentration of unlabelled patient antigen present in the test mixture, the less bound fluorescent antigen is present and, consequently, the lower the polarisation of the fluorescent light emission. Standard calibration yields quantitative results. (05 Mar 2000) |
| fluorescence polarization | Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction. (12 Dec 1998) |
| fluorescence polarization immunoassay | Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labelled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations. (12 Dec 1998) |
| fluorescence recovery after photobleaching | Many fluorochromes are bleached by exposure to exciting light. If, for example: the cell surface is labelled with a fluorescent probe and an area bleached by laser illumination, then the bleached patch that starts off as a dark area will gradually recover fluorescence. The recovery is due to the re population of the area by unbleached molecules and diffusion of bleached molecules to other areas. The rate and extent of recovery are a measure of the fluidity of the membrane and the proportion of labelled molecules that are free to exchange with adjacent areas. The technique is usually applied to cell surface fluidity or viscosity measurements, but is also applicable to other structures. (18 Nov 1997) |
| fluorescence spectrum | Fluorescence evoked over a range of wavelengths when the excitation wavelength is at a maximum. (05 Mar 2000) |