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aseptic technique <procedure> A method used by microbiologists and clinicians to keep cultures, sterile instruments and media, and people free of microbial contamination.
(09 Oct 1997)
atrial-well technique An obsolete semi-closed surgical technique for repairing atrial septal defects and other cardiac abnormalities.
(05 Mar 2000)
attachment apparatus The tissues that attach the tooth to the alveolar process: cementum, periodontal membrane, and alveolar bone.
(05 Mar 2000)
Barcroft-Warburg apparatus An apparatus for measuring the oxygen consumption of incubated tissue slices by manometric measurement of changes in gas pressure produced by oxygen absorption in an enclosed flask.
Synonym: Barcroft-Warburg apparatus.
(05 Mar 2000)
Barcroft-Warburg technique An apparatus for measuring the oxygen consumption of incubated tissue slices by manometric measurement of changes in gas pressure produced by oxygen absorption in an enclosed flask.
Synonym: Barcroft-Warburg apparatus.
(05 Mar 2000)
Beckmann's apparatus Apparatus for the accurate measurement of melting points and boiling points in connection with molecular weight determinations.
(05 Mar 2000)
Begg light wire differential force technique An orthodontic appliance utilizing small gauge labial wires with expansion and contraction loops formed into it and attached to bands fitted to individual teeth; sometimes called Begg light wire differential force technique.
(05 Mar 2000)
Benedict-Roth apparatus <apparatus, physiology> A device employed to measure the amount of oxygen utilised in quiet breathing in the basal state for the estimation of the basal metabolic rate; the subject rebreathes oxygen through soda lime from a recording spirometer.
(14 Aug 2000)
Berk Sharp technique <molecular biology, procedure> A technique of genetic mapping in which mRNA is hybridised with single stranded DNA and the nonhybridised DNA then digested with S1 nuclease, the residual DNA that hybridised with the messenger is then characterised by restriction mapping.
(18 Nov 1997)
bone demineralization technique Removal of mineral constituents or salts from bone or bone tissue. Demineralization is used as a method of studying bone strength and bone chemistry.
(12 Dec 1998)
branchial apparatus The aggregate of the pharyngeal arches, pouches, clefts, and membranes seen in the developing embryo of vertebrates.
(05 Mar 2000)
radioisotope dilution technique Method for assessing flow through a system by injection of a known quantity of radionuclide into the system and monitoring its concentration over time at a specific point in the system.
(12 Dec 1998)
Van Slyke apparatus An apparatus for determining the amounts of respiratory gases in the blood.
(05 Mar 2000)
random amplified polymorphic DNA technique Technique that utilises low-stringency polymerase chain reaction (pcr) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. Rapd technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.
(12 Dec 1998)
PAP technique 1. <gynaecology> Colloquial abbreviation for Papanicolaou's stain.
2. <technique> Peroxidase antiperoxidase method for obtaining an enhanced peroxidase reaction to indicate antibody binding to antigen.
In the first stage the material, for example a section, is reacted with a specific antiserum (say rat) against the antigen. In the next stage a large excess of say rabbit antirat immunoglobulin is applied so that only one of the binding sites is bound to the first antibody.
Then a rat antiperoxidase antiserum is bound to the second antibody unfilled sites and finally peroxidase is added and binds to the third antiserum before the peroxidase is used to develop a colour reaction.
(18 Nov 1997)
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