| high-resolution banding | Banding, especially in prophase, which increases the clarity and number of discernible chromosome bands. (05 Mar 2000) |
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| pulmonary artery banding | A surgical method of decreasing pulmonary blood flow and thereby volume overload of the left ventricle, alleviating CHF in certain congenital heart defects. (05 Mar 2000) |
| NOR-banding | A procedure which utilises a silver stain that preferentially accumulates in the nucleoli-organizing regions, i.e., the satellite regions of the acrocentric chromosomes. (05 Mar 2000) |
| Q-banding | <cell biology, technique> A fluorescent stain for chromosomes which produces specific banding patterns for each pair of homologous chromosomes. The acridine dye derivative, quinacrine hydrochloride, or other derivatives like quinacrine mustard dihydrochloride produces a green-yellow fluorescence at pH 4.5 in chromosome segments rich in constitutive heterochromatin with deoxyadenylate-deoxythymidilate (A-T) bases of DNA. The centromeric regions of human chromosomes 3, 4, and 13 are specifically stained, as are satellites of some acrocentric chromosomes and the end of the long arm of the Y chromosome. Banding patterns are similar to those obtained with G-banding stain and similar fluorescent stain results are seen with the antibiotics adriamycin and daunomycin, as well as the tertiary dyes butyl proflavine and dapi, and the bisbenzimidazole dye hoechst 33258. See: banding patterns, Q bands, quinacrine. Synonym: quinacrine chromosome banding stain. (12 Jul 2000) |
| quinacrine chromosome banding stain | q-banding stain |
| telomeric R-banding stain | <technique> A modified R-banding stain in which the telomeres become strongly stained and faint R-banding still occurs over the rest of the chromosomes; uses air-dried slides, aging for several days, and staining in hot phosphate-buffered Giemsa stain. (05 Mar 2000) |
| Trusler's rule for pulmonary artery banding | A method that gives guidance as to the correct tightness of the band; the degree of banding for a complex congenital cardiac anomaly with bidirectional shunting less than that for simple ones. (05 Mar 2000) |
| trypsin G-banding stain | <technique> A unique chromosome staining technique, used in human cytogenetics to identify individual chromosomes, which produces characteristic bands. It utilises acetic acid fixation, air drying, denaturing chromosomes mildly with proteolytic enzymes, salts, heat, detergents, or urea, and finally Giemsa stain; chromosome bands appear similar to those fluorochromed by Q-banding stain. Synonym: Giemsa chromosome banding stain. (05 Mar 2000) |
| Abbott's method | A method of treatment of scoliosis by use of a series of plaster jackets applied after partial correction of the curvature by external force. (05 Mar 2000) |
| Abell-Kendall method | A standard method for estimation of total serum cholesterol involving saponification of cholesterol ester by hydroxide, extraction with petroleum ether, and colour development with acetic anhydride-sulfuric acid; the method avoids interference by bilirubin, protein, and haemoglobin. (05 Mar 2000) |
| activated sludge method | A method of sewage disposal in which the sewage is treated with 15% bacterially active, liquid sludge, which is produced by repeated vigorous aeration of fresh sewage to form floccules or sediment; when this flocculation process is complete, the resulting activated sludge contains large numbers of bacteria, together with yeasts, molds, and protozoa, which actively effect the oxidation of organic compounds; this mixture is piped to a sedimentation tank, the effluent from which is completely treated sewage. (05 Mar 2000) |
| Altmann-Gersh method | The method of rapidly freezing a tissue and dehydrating it in a vacuum. (05 Mar 2000) |
| Anel's method | Ligation of an artery immediately above (on the proximal side of) an aneurysm. (05 Mar 2000) |
| Antyllus' method | Ligation of the artery above and below an aneurysm, followed by incision into and emptying of the sac. (05 Mar 2000) |
| aristotelian method | A method of study that stresses the relation between a general category and a particular object. (05 Mar 2000) |