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melzer's reagent A stain used to study the cellular structure of fungi.
(09 Oct 1997)
Gunzberg's reagent Phloroglucin and vanillin used as a reagent in Gunzberg's test.
(05 Mar 2000)
Meyer's reagent A solution of phenolphthalein 0.032, in decinormal sodium hydroxide 21, with water (distilled from glass) sufficient to make 100; in the presence of minute traces of blood, the solution becomes purple or blue-red.
(05 Mar 2000)
Rosenthaler-Turk reagent A solution of potassium arsenate in sulfuric acid used in obtaining colour tests for various opium alkaloids.
(05 Mar 2000)
Millon's reagent Mercuric nitrate and nitric acid as used in the Millon reaction.
(05 Mar 2000)
Cleland's reagent <chemical> A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of sh (thiol) groups and for reducing disulphides to dithiols.
Pharmacological action: sulfhydryl reagents, expectorants.
Chemical name: 2,3-Butanediol, 1,4-dimercapto-, (R*,R*)-
(12 Dec 1998)
Wurster's reagent Filter paper impregnated with tetramethyl-p-phenylenediamine, which turns blue in the presence of ozone or hydrogen peroxide.
(05 Mar 2000)
cross-linking reagent <chemistry> Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
(12 Dec 1998)
Hahn's oxine reagent An alcoholic solution of 8-hydroxyquinoline used in the determination of zinc, aluminum, magnesium, etc.
(05 Mar 2000)
Hammarsten's reagent A mixture of 1 part 25% solution of nitric acid and 19 parts 25% solution of hydrochloric acid; the addition of a few drops to a mixture of 1 part of this reagent and 4 parts alcohol will give a green colour if bile is present.
(05 Mar 2000)
Sanger's reagent A reagent used to combine with the free NH2 group of the NH2-terminal amino acid residue in a peptide, thus marking this residue; the combined forms are known as DNP-proteins, Dnp-aminoacyl, etc., the fluorine having been replaced to leave a dinitrophenyl residue (DNP, Dnp, or N2Ph-) attached to the NH2 group.
Synonym: Sanger's reagent.
Acronym: FDNB
(05 Mar 2000)
Schaer's reagent An alcoholic or aqueous solution of chloral hydrate used as an extraction medium in investigations of alkaloids.
(05 Mar 2000)
Scheibler's reagent A solution of sodium tungstate in phosphoric acid used in tests for alkaloids.
(05 Mar 2000)
Schiff's reagent An aqueous solution of basic fuchsin or pararosaniline which is decolorised by sulfur dioxide, commonly prepared by addition of hydrochloric acid to a dye solution containing a metabisulphite or bisulphite salt; used for aldehydes and in histochemistry to detect polysaccharides, DNA, and proteins.
See: Feulgen stain, periodic acid-Schiff stain, ninhydrin-Schiff stain for proteins.
(05 Mar 2000)
Scott-Wilson reagent An alkaline solution of mercuric cyanide and silver nitrate used in the detection of acetone.
(05 Mar 2000)
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