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restraint 1. The act or process of restraining, or of holding back or hindering from motion or action, in any manner; hindrance of the will, or of any action, physical or mental. "No man was altogether above the restrains of law, and no man altogether below its protection." (Macaulay)
2. The state of being restrained.
3. That which restrains, as a law, a prohibition, or the like; limitation; restriction. "For one restraint, lords of the world besides." (Milton)
Synonym: Repression, hindrance, check, stop, curb,oercion, confinement, limitation, restriction.
Origin: OF. Restraincte, fr. Restrainct, F. Restreint, p. P. Of restraindre, restrendre. See Restrain.
Source: Websters Dictionary
(01 Mar 1998)
restraint, physical Use of a device for the purpose of preventing the individual from moving all or part of the body. The concept excludes splints and casts.
(12 Dec 1998)
restriction 1. The process with which foreign DNA that has been introduced into a prokaryotic cell becomes ineffective.
2. A limitation.
(05 Mar 2000)
restriction endonuclease <enzyme, molecular biology> Class of bacterial enzymes that cut DNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages (host DNA is specifically modified at these sites).
Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site.
Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends.
They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. Coli are designated Eco RI and Eco RII.
(10 Mar 1998)
restriction enzyme <enzyme, molecular biology> Class of bacterial enzymes that cut DNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages (host DNA is specifically modified at these sites).
Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site.
Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends.
They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. Coli are designated Eco RI and Eco RII.
(10 Mar 1998)
restriction enzyme cutting site <molecular biology> A specific nucleotide sequence of DNA at which a particular restriction enzyme cuts the DNA.
Some sites occur frequently in DNA (for example, every several hundred basepairs), others much less frequently (rare-cutter, for example, every 10,000 base pairs).
(10 Mar 1998)
restriction enzyme, endonuclease A protein that recognises specific, short nucleotide sequences and cuts DNA at those sites. Bacteria contain over 400 such enzymes that recognise and cut over 100 different DNA sequences. See restriction enzyme cutting site.
(05 Mar 2000)
restriction fragment <molecular biology> The fragments of DNA generated by digesting DNA with a specific restriction endonuclease. Each of the fragments ends in a site recognised by that specific enzyme.
(10 Mar 1998)
restriction fragment length polymorphism <molecular biology, technique> A method that allows familial relationships to be established by comparing the characteristic polymorphic patterns that are obtained when certain regions of genomic DNA are amplified (typically by PCR) and cut with certain restriction enzymes.
The variation in the length of DNA fragments produced by a restriction endonuclease that cuts at a polymorphic locus. Such variations are generated by mutations that create or abolish recognition sites for these enzymes.
This is a key tool in DNA fingerprinting, reflecting the existence of different alleles in the individual. Restriction fragment length polymorphism mapping is also used in plant breeding to see if a key trait such as disease resistance is inherited.
In principle, an individual can be identified unambiquously by restriction fragment length polymorphism hence the use of restriction fragment length polymorphism in forensic analysis of blood, hair or semen).
Similarly, if a polymorphism can be identified close to the locus of a genetic defect, it provides a valuable marker for tracing the inheritance of the defect.
Synonym: DNA fingerprinting.
Acronym: RFLP
(12 Jan 1998)
restriction length polymorphism Fragment length polymorphism, the existence of allelic forms recognizable by the length of fragments that result when the nucleotide chain is treated by a specific restriction enzyme that cleaves wherever a particular sequence of nucleotides occurs. A mutation in this sequence changes cleaving and hence the number of fragments.
(05 Mar 2000)
restriction map <molecular biology> Map of DNA showing the position of sites recognised and cut by various restriction endonucleases.
(12 Jan 1998)
restriction mapping Use of restriction endonucleases to analyze and generate a physical map of genomes or genes. The nucleotide sequence determined is often then translated into an amino acid sequence, providing a means for sequencing the protein for which the gene codes, or for which the mRNA is a messenger.
(12 Dec 1998)
restriction methylation The enzymatic addition of methyl groups to selected adenine and cytosine residues to protect from hydrolysis by certain restriction enzymes.
(05 Mar 2000)
restriction nuclease <enzyme, molecular biology> Class of bacterial enzymes that cut DNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages (host DNA is specifically modified at these sites).
Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site.
Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends.
They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. Coli are designated Eco RI and Eco RII.
(10 Mar 1998)
restriction site A sequence in DNA that can be recognised and cut by a specific restriction enzyme.
(12 Dec 1998)
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resting spore A thick-walled spore, particularly one formed by a sexual process, germinating only after an extended period of dormancy (eg, an overwintering teliospore). (16)
Ãâó: ppathw3.cals.cornell.edu/glossary/Defs_R.htm
resilient having the property of springing back.
Ãâó: www.biology.lsu.edu/heydrjay/ThomasSay/terms.html
restriction enzyme Enzyme that recognizes specific nucleotide sequences in DNA and cuts the DNA molecule at these points.
Ãâó: science.education.nih.gov/supplements/nih1/genetic...
resection Surgery to remove tissue, an organ, or part of an organ.
Ãâó: www.cdc.gov/cancer/lung/glossary.htm
resection A term used to describe the removal of a tumor from an organ such as a kidney, while retaining (sparing) the organ itself.
Ãâó: aspin.asu.edu/geneinfo/glos-r.htm
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RES disposed to or engaged in defiance of established authority
RES exhibiting or relating to electrical resistance
RES a material's opposition to the flow of electric current
RES offering no resistance
RES impossible to resist
RES an electrical device that resists the flow of electrical current
RES change the size of
RES smudge again
RES put a new sole on
RES characterized by quickness and firmness
RES firm in purpose or belief
RES showing firm determination or purpose
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